RAPAd® Universal Adenoviral Expression System

  • Catalog number
    VPK-250
  • Price
    Please ask
  • Size
    1 test kit
  • We send this reagant on
    Blue Ice
  • The temperatureerature for storage is
    -20ºC
  • SDS or safty data sheet
    more (VPK-250, 251, 252, 253, 254, 255, 256) GHS.pdf
  • Type of reagent
    Viral Expression
  • Related techniques
    Viral Expression, Adenoviral Expression, Adenoviral Expression Systems
  • Use of the reagent
    Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed. The RAPAd® Universal Adenoviral Expression System (# VPK-250) contains a promoterless shuttle vector to allow you to insert the promoter of your choice along with your gene of interest to maximize gene expression.
  • Cellular use
    Engineered to produce 100- to 10,000-fold fewer wild type plaques than most other expression systems
  • Protocol information
    Generate high-titer virus in about 2-3 weeks compared to 2-3 months with other systems
  • Reagent properties
    Promoterless shuttle vector allows you to clone the optimal promoter with your gene of interest
  • Additional lab advice
    Cloning capacity of shuttle vector = 7.5 kb
  • Living modified organisme in this reagent or LMO
    Not applicable
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,70
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
MeSH Data
  • Name
  • Concept
    Scope note: Process of growing viruses in live animals, plants, or cultured cells.
  • Tree numbers
    • E01.370.225.875.970
    • E05.200.875.970
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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