RAPAd® CMV Adenoviral Expression System
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Catalog number
VPK-252
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Price
Please ask
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Size
1 test kit
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We send this reagant on
Blue Ice
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The temperatureerature for storage is
-20ºC
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SDS or safty data sheet
more (VPK-250, 251, 252, 253, 254, 255, 256) GHS.pdf
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Type of reagent
Viral Expression
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Related techniques
Viral Expression, Adenoviral Expression, Adenoviral Expression Systems
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Use of the reagent
Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.
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Cellular use
Engineered to produce 100- to 10,000-fold fewer wild type plaques than most other expression systems
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Protocol information
Generate high-titer virus in about 2-3 weeks compared to 2-3 months with other systems
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Reagent properties
Cloning capacity of shuttle vector = 6.9 kb
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Living modified organisme in this reagent or LMO
Not applicable
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,72
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
MeSH Data
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Name
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Concept
Scope note:
Process of growing viruses in live animals, plants, or cultured cells.
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Tree numbers
- E01.370.225.875.970
- E05.200.875.970
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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