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Lysates properties
Raji (Human lymphoma; B lymphoma) Cell cytoplasmic protein lysate cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Raji (Human lymphoma; B lymphoma) cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Raji (Human lymphoma; B lymphoma) tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
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Lysate s origin
Cell Line
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State of tissue
Diseased
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Species of the lysate
Human
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Cellular origibn of the lysate
Raji (Human Lymphoma; B Lymphoma)
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Disease of the lysate
Tumor
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Localisation of the biopsy
Raji (Human Lymphoma; B Lymphoma) Cell Line
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Ug of lysate used by test
Raji (Lymphoma; B Lymphoma) Cell Cytoplasmic protein lysate is for use in Western blotting, 10 ug to 20 ug per lane is recommended for mini gel.
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Positive control in
the lysate is used as positive control in Western Blot
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PH and medium
HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
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Keep at
Store at 2-8 grades C for continuous use. For extended storage, freeze working aliquots at -70 grades C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
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Caution
This product is for research use only.
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Laboratory requirements
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. GENTAUR is not liable for damages or injuries resulting from receipt and/or use of GENTAUR materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs).
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Animal origin
Human
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Dilution
2 mg/mL or more concentrated
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