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Alternative name1
Anti-RABBIT ANTI HUMAN RAD52
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Alternative name2
RABBIT ANTI HUMAN RAD52
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Alternative name3
Lapin ANTI Homo sapiens RAD52
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Alternative name4
RAD52
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Alternative name5
N/A
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Other names
DNA repair protein RAD52 homolog; DNA repair protein RAD52 homolog; DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23; RAD52 homolog (S. cerevisiae); N/A
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Gene name
RAD52
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Gene name synonims
N/A
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Other gene names
RAD52; RAD52; N/A; N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
Polyclonal IgG
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Clone
Polyclonal antibody
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Host organism
Rabbit
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Species reactivity
Human
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Specificity and cross reactivity
RAD52 This item recognises Human Rad52, a protein which functions in DNA repair through the homologous recombination of DNA double strand breaks (DSBs). DSBs can be generated through exposure to ionizing radiation or by endogenous cellular reactions, and Rad52 acts during the latter stages of recombination after the DSBs are processed into single stranded DNA (ssDNA). _x000D__x000D_Rad52 functions together with Rad51 and RPA (Replication Protein A), where it has two distinct activities. First, Rad52 and RPA form a co-complex on ssDNA, where Rad52 functions to recruit Rad51. In addition, Rad52 has ssDNA binding and annealing activities, particularly ssDNA saturated with RPA. _x000D__x000D_Rad52 is present at low levels during the G1 phase of the cell cycle, when recombinatorial repair is absent. The level rises steadily through S phase reaching a maximum in G2 phase when homologous repair is also at its highest level.
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Purification method
N/A
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Form Appearance
Purified (Purified IgG - liquid)
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Concentration
N/A
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Storage and shipping
Store at +Store productone at +4 degrees Celsius. or productone should be stored at -20 degrees Celsius. if preferred. Storage in frost-free freezers is not recommended. productone should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should productone contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of dispatch.
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Tested for
Immunohistology - Paraffin (IHC), ELISA (EIA), Western Blot (WB)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
MBS Polyclonals supplies antibodies that are for research of human proteins.
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French translation
anticorps
