RAC1(Ser71) Antibody, ALEXA FLUOR 350
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Catalog number
bs-5698R-A350
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Price
Please ask
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Size
100ul
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Long name
RAC1(Ser71) Polyclonal Antibody, ALEXA FLUOR 350 Conjugated
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Also known as
Anti-RAC1 Ser71 PAb ALEXA FLUOR 350
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 350
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
RAC1 Ser71
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Specificity
This is a highly specific antibody against RAC1 Ser71.
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Modification
Phosphorylation
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Modification Site
Ser71
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
60-75/192
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Subcellular location
Cytoplasm
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Source
KLH conjugated synthetic phosphopeptide derived from human RAC1 around the phosphorylation site of Ser71
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Gene ID Number
5879
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Swiss Prot
P63000
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Tested applications
IF(IHC-P), IF(ICC)
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Recommended dilutions
IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
343nm/442nm
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Synonyms
MIG5; Rac-1; TC-25; p21-Rac1; Ras-related C3 botulinum toxin substrate 1; Cell migration-inducing gene 5 protein; Ras-like protein TC25; RAC1; TC25
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor, ALEXA FLUOR 350
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French translation
anticorps
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Gene target
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Gene symbol
RAC1, RNASE1
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Short name
Anti-RAC1 Ser71
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 350
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Alternative name
RAC1(Ser71) Polyclonal Antibody, ALEXA FLUOR 350
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Alternative technique
antibodies
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Alternative to gene target
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), p21-Rac1 and Rac-1 and TC-25, RAC1 and IDBG-8115 and ENSG00000136238 and 5879, Rho GDP-dissociation inhibitor binding, Plasma membranes, Rac1 and IDBG-207763 and ENSMUSG00000001847 and 19353, RAC1 and IDBG-641023 and ENSBTAG00000009233 and 281440
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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