Rabbit Polyclonal IgG Anti-VEGF-A, murine

  • Catalog number
    VEGA03
  • Price
    Please ask
  • Size
    100 µg
  • Availability
    Please contact us to check the availability
  • Immunogen
    Highly purified recombinant murine VEGF164
  • Specificity
    VEGF-A isoforms in mouse and humans (Western Blot under reducing and non-reducing conditions)
  • Species reactivity
    Human, Mouse
  • Product presentation
    Protein A purified IgG lyophilised from PBS pH 7.4 without preservative
  • Product category
    Primary Antibody
  • Antibody s raised in
    Rabbit
  • Antibody s isotype
    IgG
  • Antibody s clone
    polyclonal
  • Product is supplied as
    lyophilisate
  • Recommended concentration for use
    Direct ELISA 0.5-5 µg/ml, Western Blot 0.1-0.2 µg/ml, IHC 0.5-2 µg/ml
  • Protein concentration
    100 µg
  • Positive control
    Metastasing tumours of different origin
  • Pretreatment
    Formaldehyde fixed tissue shows good results after pre-treatment with proteinase K (0.01%, 15 min at RT), other proteases and micro-wave techniques are not validated.
  • Tested applications
    IHC(C, P), ELISA, WB
  • Recommended secondary reagent
    As secondary reagents we recommend the VECTASTAIN ABC Elite Kit Rabbit IgG (Ref. PK-6101) or ImmPRESS anti-Rabbit IgG (Ref. MP-7401)
  • Storage method
    Store at -20°C
  • UniProt number
    QGo to UniProt/SwissProtGo to UniProt/SwissProt731 (VEGFA_MOUSE)
  • Tips
    Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Group
    Polyclonals and antibodies
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    VEGF-A   murine  
  • Gene symbol
    VEGFA, VEGFC, VEGFD, HLA-A, IGHV5-10-1, CIDEA, RPA1, CDC37P1, OR1Q1
  • Short name
    Research
  • Technique
    Polyclonal, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG, IgG
  • Alternative name
    VEGF-A, murine
  • Alternative technique
    polyclonals, rabbit-anti, antibodies, immunoglobulins
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 5-10-1
  • Synonyms gene
  • Synonyms gene name
    • immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
    • immunoglobulin heavy variable 5-A (provisional)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-04
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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