Rabbit Polyclonal IgG Anti-VEGF-A, murine
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Catalog numberVEGA03
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PricePlease ask
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Size100 µg
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AvailabilityPlease contact us to check the availability
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ImmunogenHighly purified recombinant murine VEGF164
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SpecificityVEGF-A isoforms in mouse and humans (Western Blot under reducing and non-reducing conditions)
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Species reactivityHuman, Mouse
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Product presentationProtein A purified IgG lyophilised from PBS pH 7.4 without preservative
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Product categoryPrimary Antibody
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Antibody s raised inRabbit
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Antibody s isotypeIgG
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Antibody s clonepolyclonal
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Product is supplied aslyophilisate
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Recommended concentration for useDirect ELISA 0.5-5 µg/ml, Western Blot 0.1-0.2 µg/ml, IHC 0.5-2 µg/ml
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Protein concentration100 µg
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Positive controlMetastasing tumours of different origin
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PretreatmentFormaldehyde fixed tissue shows good results after pre-treatment with proteinase K (0.01%, 15 min at RT), other proteases and micro-wave techniques are not validated.
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Tested applicationsIHC(C, P), ELISA, WB
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Recommended secondary reagentAs secondary reagents we recommend the VECTASTAIN ABC Elite Kit Rabbit IgG (Ref. PK-6101) or ImmPRESS anti-Rabbit IgG (Ref. MP-7401)
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Storage methodStore at -20°C
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UniProt numberQGo to UniProt/SwissProtGo to UniProt/SwissProt731 (VEGFA_MOUSE)
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TipsGentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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GroupPolyclonals and antibodies
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolVEGFA, VEGFC, VEGFD, HLA-A, IGHV5-10-1, CIDEA, RPA1, CDC37P1, OR1Q1
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Short nameResearch
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TechniquePolyclonal, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeIgG, IgG
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Alternative nameVEGF-A, murine
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Alternative techniquepolyclonals, rabbit-anti, antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namevascular endothelial growth factor A
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Synonyms gene
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Synonyms gene name
- vascular endothelial growth factor
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-01-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- VEGF family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namevascular endothelial growth factor C
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-10-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- VEGF family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namevascular endothelial growth factor D
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Synonyms gene
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Synonyms gene name
- c-fos induced growth factor (vascular endothelial growth factor D)
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-03-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- VEGF family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication protein A1
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Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nucleotide excision repair
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell division cycle 37 pseudogene 1
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Synonyms gene name
- cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
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Synonyms
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Locus
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Discovery year2009-01-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily Q member 1
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 1, subfamily Q, member 1
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Synonyms
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Locus
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Discovery year1999-12-09
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Entrez gene record
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Classification
- Olfactory receptors, family 1
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data