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Availability
Please contact us to check the availability
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Immunogen
The immunizing peptide corresponds to amino acid residues 362-380 from rat RAGE, Synthetic Peptide: C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(NH2)(380). This sequence is 100% conserved in mouse and 70% conserved in humans.
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Specificity
Rat and mouse receptor for Advanced Glycation End Products
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Species reactivity
Rat, mouse, others not tested
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Product presentation
Peptide affinity purified antibody in PBS, 0,1% BSA, 0,05% NaN3
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
IgG
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Antibody s clone
polyclonal
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1-5 µg/ml IHC, 1-2 µg/ml WB
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Protein concentration
1 mg/ml
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Positive control
Diabetic retina or renal tissue
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Pretreatment
Immunohistochemistry in paraffin sections has not been established yet. The application of Unmasking Fluid G (Ref. DE007) at 100°C may be advantageous.
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Tested applications
IHC(C), WB
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Recommended secondary reagent
Recommended secondary systems in IHC are our Universal Staining Kits Ref. AE005 or DA005 or the VECTASTAIN Elite ABC System Rabbit IgG (Ref. PK-6101). The latter is also suitable for Western Blotting or other techniques.
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Storage method
Store at -20°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Schmidt, A.M. et al. (1996) RAGE: a novel cellular receptor for advanced glycation end products. Diabetes 45; 577-580. 2. Durr E, Yu J, Krasinska KM, Carver LA, Yates JR, Testa JE, Oh P, Schnitzer JE. Direct proteomic mapping of the lung microvascular endothelial cell surface in vivo and in cell culture. Nat Biotechnol. 2004 Aug;22(8):985-92..
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Tips
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
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Group
Polyclonals and antibodies
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Gene target
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Gene symbol
AGER, MOK
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Short name
AGE
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Technique
Polyclonal, Rabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Species
Mouse, Mouses, Rats
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Alternative name
RAGE, Rat/Mouse, Receptor for Advanced Glycation End Products
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Alternative technique
polyclonals, rabbit-anti, murine, antibodies, immunoglobulins
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