Rabbit Polyclonal IgG Anti-NF kappa B / p65

• Catalog number
  NFK001
• Price
  Please ask
• Size
  100 µg

• Availability
  Please contact us to check the availability
• Immunogen
  Synthetic peptide from internal domain of NFkB p65
• Specificity
  NFkB (65kDa)
• Species reactivity
  Human, mouse, rat (other vertebrates expected, but not tested)
• Product presentation
  Affinity purified IgG fraction from rabbit serum in PBS containing 0.2% BSA and 0.09% NaN3
• Product category
  Primary Antibody
• Antibody s raised in
  Rabbit
• Antibody s isotype
  IgG
• Antibody s clone
  polyclonal
• Product is supplied as
  liquid concentrate
• Recommended concentration for use
  IHC (2 µg/ml), WB (2-5 µg/ml)
• Protein concentration
  200 µg/ml
• Positive control
  Prostate, placenta
• Pretreatment
  Unmasking with citrate buffer pH 6 10-20 min at 100°C
• Tested applications
  IHC(P), WB
• Recommended secondary reagent
  Biotinylated anti-Rabbit IgG (Art. No. ZU101) in combination with Streptavidin:HRPO (Art. No. ZU054) or Streptavidin:AP (Art. No. ZU051) and a suitable substrate.
• Storage method
  Store at 2-8°C
• UniProt number
  Go to UniProt/SwissProt
• Tips
  *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• Other products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Group
  Polyclonals and antibodies
• Latin name
  Oryctolagus cuniculus

• Gene target
  kappa   p65  
• Gene symbol
  RELA
• Short name
  Research
• Technique
  Polyclonal, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  IgG, IgG
• Alternative name
  NF kappa B / p65
• Alternative technique
  polyclonals, rabbit-anti, antibodies, immunoglobulins

Gene info

• Identity
  HGNC:9955
• Gene
  RELA
• Long gene name
  RELA proto-oncogene, NF-kB subunit
• Synonyms gene
  • NFKB3
• Synonyms gene name
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
• Synonyms
  • p65
• GenBank acession
  • Z22951
• Locus
  11q13.1
• Discovery year
  1991-11-14
• Entrez gene record
  5970
• Pubmed identfication
  • 2001591
• RefSeq identity
  • NM_021975
• Classification
  • NF-kappa B complex subunits
  • IPT domain containing
• VEGA ID
  OTTHUMG00000166566

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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