Rabbit Polyclonal IgG Anti-Laminin, human
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Catalog number
LA24811
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Price
Please ask
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Size
0.5 ml
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Availability
Please contact us to check the availability
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Immunogen
Purified laminin P1 fragment from human placenta
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Specificity
Human laminin fragment P1 100%, human collagen type I and IV
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Species reactivity
Human
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Product presentation
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
IgG
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Antibody s clone
polyclonal
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Product is supplied as
purified, lyophilized
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Recommended concentration for use
IFA ≥ 1:80, IHC(P) ≥ 1:250, ELISA ≥ 1:200 (OD ≥ 500)
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Protein concentration
app. 1 mg/ml
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Positive control
Human skin
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Pretreatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
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Tested applications
IFA, IHC(P), ELISA, RIA
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Recommended secondary reagent
Anti-rabbit IgG-conjugates
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Storage method
Store at -20°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
See list of references in BIOLOGO ECM brochure.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
LAMB2, LAMA5, LAMA4
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Short name
ECM
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Technique
Polyclonal, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Species
Human, Humans
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Alternative name
Laminin, human
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Alternative technique
polyclonals, rabbit-anti, antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
laminin subunit beta 2
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Synonyms gene
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Synonyms gene name
- laminin, beta 2 (laminin S)
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Synonyms
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Synonyms name
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Locus
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Discovery year
1992-05-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
laminin subunit alpha 5
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Synonyms gene name
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GenBank acession
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Locus
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Discovery year
1997-10-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Laminin subunits
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
laminin subunit alpha 4
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Synonyms gene name
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Synonyms
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Locus
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Discovery year
1993-07-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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