Rabbit Polyclonal IgG Anti-Laminin, human

• Catalog number
  LA24811
• Price
  Please ask
• Size
  0.5 ml

• Availability
  Please contact us to check the availability
• Immunogen
  Purified laminin P1 fragment from human placenta
• Specificity
  Human laminin fragment P1 100%, human collagen type I and IV
• Species reactivity
  Human
• Product presentation
  Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
• Product category
  Primary Antibody
• Antibody s raised in
  Rabbit
• Antibody s isotype
  IgG
• Antibody s clone
  polyclonal
• Product is supplied as
  purified, lyophilized
• Recommended concentration for use
  IFA ≥ 1:80, IHC(P) ≥ 1:250, ELISA ≥ 1:200 (OD ≥ 500)
• Protein concentration
  app. 1 mg/ml
• Positive control
  Human skin
• Pretreatment
  After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Tested applications
  IFA, IHC(P), ELISA, RIA
• Recommended secondary reagent
  Anti-rabbit IgG-conjugates
• Storage method
  Store at -20°C
• UniProt number
  Go to UniProt/SwissProt
• Litterature
  See list of references in BIOLOGO ECM brochure.
• Tips
  *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
• Other products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Group
  Polyclonals and antibodies
• Latin name
  Oryctolagus cuniculus

• Gene target
  Laminin  
• Gene symbol
  LAMB2, LAMA5, LAMA4
• Short name
  ECM
• Technique
  Polyclonal, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  IgG, IgG
• Species
  Human, Humans
• Alternative name
  Laminin, human
• Alternative technique
  polyclonals, rabbit-anti, antibodies, immunoglobulins

Gene info

• Identity
  HGNC:6487
• Gene
  LAMB2
• Long gene name
  laminin subunit beta 2
• Synonyms gene
  • LAMS
• Synonyms gene name
  • laminin, beta 2 (laminin S)
• Synonyms
  • NPHS5
• Synonyms name
  • laminin S
• Locus
  3p21.31
• Discovery year
  1992-05-06
• Entrez gene record
  3913
• Pubmed identfication
  • 2922051
  • 10393422
• RefSeq identity
  • NM_002292
• Classification
  • Laminin subunits
• VEGA ID
  OTTHUMG00000156807

Gene info

• Identity
  HGNC:6485
• Gene
  LAMA5
• Long gene name
  laminin subunit alpha 5
• Synonyms gene name
  • laminin, alpha 5
• GenBank acession
  • AF443072
• Locus
  20q13.33
• Discovery year
  1997-10-30
• Entrez gene record
  3911
• Pubmed identfication
  • 9271224
• RefSeq identity
  • NM_005560
• Classification
  • Laminin subunits
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000032908

Gene info

• Identity
  HGNC:6484
• Gene
  LAMA4
• Long gene name
  laminin subunit alpha 4
• Synonyms gene name
  • laminin, alpha 4
• Synonyms
  • LAMA3
• Locus
  6q21
• Discovery year
  1993-07-26
• Entrez gene record
  3910
• Pubmed identfication
  • 7959779
• RefSeq identity
  • NM_001105206
• Classification
  • Laminin subunits
• VEGA ID
  OTTHUMG00000015386
• Locus Specific Databases
  • LRG_433

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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