Rabbit Polyclonal IgG Anti-Collagen Type III, human

  • Catalog number
    CO20311-0.1
  • Price
    Please ask
  • Size
    0.1 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Purified collagen type III from human placenta
  • Specificity
    Human collagen type III 100% bindung in RIA at 1:40 dilution, cross-reactivity with human collagen type I and IV
  • Species reactivity
    Human strictly, no cross-reactivity with other species so far tested (cattle, swine, rat, mouse)
  • Product presentation
    affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
  • Product category
    Primary Antibody
  • Antibody s raised in
    Rabbit
  • Antibody s isotype
    IgG
  • Antibody s clone
    polyclonal
  • Product is supplied as
    purified, lyophilized
  • Recommended concentration for use
    IFA ≥ 1:80, IHC(P) ≥ 1:500, ELISA ≥ 1:200 (OD ≥ 500)
  • Protein concentration
    app. 1 mg/ml
  • Positive control
    Human skin
  • Pretreatment
    After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  • Tested applications
    IHC(P), IFA, ELISA, RIA, IB/WB
  • Recommended secondary reagent
    Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488). For paraffin sections best results have been obtained by use of DAKO Envision or VECTOR ImmPress systems (Art. No. MP-7401).
  • Storage method
    Store at -20°C
  • UniProt number
    PGo to UniProt/SwissProt2461 (CO3A1_HUMAN)
  • Litterature
    1. Black A.F., Bouez C., Perrier E., Schlotmann K., Chapuis F., Damour O. (2005) Optimization and characterization of an Engineered human skin equivalent. Tissue Engineering 11, 723-733. 2. Castera L., Hartmann D.J., Chapel F., Guettier C., Mal F., Lons T., Richardet J.P., Grimbert S., Morassi O., Beaugrand M., Trinchet J.C. (2000) Serum laminin and type IV collagen are accurate markers of histologically severe alcoholic hepatitis in patient 3. Ricard-Blum S., Hartmann D.J., Esterre Ph. (1998) Monitoring of extracellular matrix metabolism and cross-linking in tissue, serum and urine of patients with chromoblastomycosis, a chronic skin fibrosis. Eur. J. Clin. Invest. 28, 748-754.
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Gene
    Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Group
    Polyclonals and antibodies
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    Collagen   III  
  • Gene symbol
    CPLX3, MGAT3, MGST3, P4HA3, CASP5, IGHVIII-82, THAP4, MT-CSB3, IGHVIII-44, IGHVIII-67-4
  • Short name
    ECM
  • Technique
    Polyclonal, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG, IgG
  • Species
    Human, Humans
  • Alternative name
    Collagen Type III, human
  • Alternative technique
    polyclonals, rabbit-anti, antibodies, immunoglobulins
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
  • Synonyms gene name
    • mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1993-08-27
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Mannosyl-glycoprotein N-acetylglucosaminyltransferases
  • VEGA ID
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    caspase 5
  • Synonyms gene name
    • caspase 5, apoptosis-related cysteine protease
    • caspase 5, apoptosis-related cysteine peptidase
  • Synonyms
  • Locus
  • Discovery year
    1996-09-13
  • Entrez gene record
    838
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Caspases
    • Caspase recruitment domain containing
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-82 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-82
    • immunoglobulin heavy variable (III)-82 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    mitochondrially encoded conserved sequence block III
  • Synonyms gene
  • Synonyms gene name
    • conserved sequence block III
  • Synonyms
  • Locus
  • Discovery year
    1989-10-11
  • Pubmed identfication
  • Classification
    • Mitochondrially encoded regions
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-44 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-44
    • immunoglobulin heavy variable (III)-44 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-67-4 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-67-4
    • immunoglobulin heavy variable (III)-67-4 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee