Rabbit Polyclonal IgG Anti-Beta-Defensin-1 (HBD-1), affinity purified

• Catalog number
  DEF01-A
• Price
  Please ask
• Size
  100 µg

• Availability
  Please contact us to check the availability
• Immunogen
  Synthetic peptide, 17 AA near N-terminus of the mature HBD-1, coupled to KLH for immunisation of rabbits
• Specificity
  ß-defensin-1 (HBD-1) whole molecule and 17AA N-terminal fraction of HBD-1, no cross-reactivity to alpha-defensin and other beta-defensins
• Species reactivity
  human, monkey, no cross-reactivity with mouse and rat
• Product presentation
  Peptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.
• Product category
  Primary Antibody
• Antibody s raised in
  Rabbit
• Antibody s isotype
  IgG
• Antibody s clone
  polyclonal
• Product is supplied as
  lyophilisate
• Recommended concentration for use
  1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested)
• Protein concentration
  N/A
• Positive control
  Renal epithelia, bladder epithelia
• Tested applications
  IHC(C, P), ELISA, RIA
• Recommended secondary reagent
  We recommend the VECTASTAIN Elite ABC Kit Rabbit IgG (code no. PK-6101) or BioPrime Universal Staining Kits (code no. AE005 and DA005).
• Storage method
  Store at -20'°C'
• UniProt number
  Go to UniProt/SwissProt
• Litterature
  1. Bensch K.W., Raida M., Mägert H.J., Schulz-Knappe P., and W.G. Forssmann. HBD-1: a novel beta -defensin from human plasma. FEBS Lett 1995, 368; 331-335. (original publication HBD-1) 2. Valore EV, Park CH, Quayle AJ, Wiles KR, McCray PB, Ganz T. Human beta-defensin-1: an antimicrobial peptide of urogenital tissues. J Clin Invest. 1998 Apr 15;101(8):1633-42. 3. O'Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T, Kagnoff MF. Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol. 1999 Dec 15;163(12):6718-24. 4. Ganz T. Defensins and host defense. Science. 1999 Oct 15;286(5439):420-1. 5. Shi J, Zhang G, Wu H, Ross C, Blecha F, Ganz T. Porcine epithelial beta-defensin 1 is expressed in the dorsal tongue at antimicrobial concentrations. Infect Immun. 1999 Jun;67(6):3121-7.
• Tips
  Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
• Other products
• Test
  A high affinity purification column was use to purify Research by bio logo by chromatographic size exclusion.
• Description
  1 of protein A or G purified by bio logo will give more specificity than crude serum. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Group
  Polyclonals and antibodies
• Latin name
  Oryctolagus cuniculus

• Gene target
  Beta-Defensin-1   HBD-1  
• Gene symbol
  DEFB1, HBD, DEFB4A, DEFB103A, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1
• Short name
  Research
• Technique
  Polyclonal, affinity, purified, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. purifications
• Host
  Rabbit, Rabbits
• Isotype
  IgG, IgG
• Alternative name
  Beta-Defensin-1 (HBD-1), affinity purified
• Alternative technique
  polyclonals, pure, rabbit-anti, antibodies, immunoglobulins

Gene info

• Identity
  HGNC:2766
• Gene
  DEFB1
• Long gene name
  defensin beta 1
• Synonyms gene name
  • defensin, beta 1
• Synonyms
  • HBD-1
  • DEFB-1
  • DEFB101
  • HBD1
  • BD1
  • MGC51822
• Synonyms name
  • beta-defensin 1
  • beta defensin 1
• GenBank acession
  • X92744
• Locus
  8p23.1
• Discovery year
  1997-05-29
• Entrez gene record
  1672
• Pubmed identfication
  • 7628632
• RefSeq identity
  • NM_005218
• Classification
  • Defensins, beta
• VEGA ID
  OTTHUMG00000090367

Gene info

• Identity
  HGNC:4829
• Gene
  HBD
• Long gene name
  hemoglobin subunit delta
• Synonyms gene name
  • hemoglobin, delta
• GenBank acession
  • AY034468
• Locus
  11p15.4
• Discovery year
  2001-06-22
• Entrez gene record
  3045
• Pubmed identfication
  • 2649166
• RefSeq identity
  • NM_000519
• Classification
  • Hemoglobin subunits
• VEGA ID
  OTTHUMG00000066674
• Locus Specific Databases
  • HbVar: A Database of Human Hemoglobin Variants and Thalassemias
  • The Globin Gene Server
  • LRG_1335

Gene info

• Identity
  HGNC:2767
• Gene
  DEFB4A
• Long gene name
  defensin beta 4A
• Synonyms gene
  • DEFB102
  • DEFB2
  • DEFB4
• Synonyms gene name
  • defensin, beta 2
  • defensin, beta 4
  • defensin, beta 4A
• Synonyms
  • SAP1
  • HBD-2
  • DEFB-2
• Synonyms name
  • beta defensin 2
  • beta defensin-2
  • beta-defensin 2
• GenBank acession
  • AJ000152
• Locus
  8p23.1
• Discovery year
  1997-08-28
• Entrez gene record
  1673
• Pubmed identfication
  • 9202117
• RefSeq identity
  • NM_004942
• Classification
  • Receptor ligands
  • Defensins, beta
• VEGA ID
  OTTHUMG00000129314

Gene info

• Identity
  HGNC:15967
• Gene
  DEFB103A
• Long gene name
  defensin beta 103A
• Synonyms gene
  • DEFB3
  • DEFB103
• Synonyms gene name
  • defensin, beta 3
  • defensin, beta 103A
• Synonyms
  • HBD-3
  • HBP-3
  • HBD3
  • HBP3
  • DEFB-3
• Synonyms name
  • beta defensin 3
  • beta defensin-3
  • beta-defensin 3
• GenBank acession
  • AF217245
• Locus
  8p23.1
• Discovery year
  2001-06-25
• Entrez gene record
  414325
• Pubmed identfication
  • 11223260
  • 11085990
• RefSeq identity
  • NM_001081551
• Classification
  • Defensins, beta
• VEGA ID
  OTTHUMG00000143855

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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