Rabbit PCNA(Proliferating Cell Nuclear Antigen) EIA Kit
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Catalog number
E-EL-RB2132-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Rabbit
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rabbit PCNA. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit PCNA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit PCNA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit PCNA. You can calculate the concentration of Rabbit PCNA in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.94ng/mL
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Detection Range
1.56~100ng/mL
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Target Name
PCNA
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UNIProt ID
NA
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Rabbit PCNA(Proliferating Cell Nuclear Antigen) EIA Kit
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Type
Sandwich
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Description
Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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About
Rabbits are used for polyclonal antibody production by Elabscience. Rabbit antibodies are very stable and can be stored for several days at room temperature. Elabscience adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
PCNA, PCNA-AS1
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Short name
Rabbit PCNA(Proliferating Antigen) EIA Kit
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Technique
antigen, Rabbit, EIA, antigenes, eia
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Host
Rabbit, Rabbits
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Alternative name
production species: rabbit PCNA(Proliferating cellular Nuclear protein) EIA reagent
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Alternative technique
antigenes, rabbit-anti, kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell
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Gene info
Gene info
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Identity
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Gene
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Long gene name
PCNA antisense RNA 1
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Synonyms gene
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Synonyms gene name
- PCNA antisense RNA (non-protein coding)
- PCNA antisense RNA 1 (non-protein coding)
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Locus
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Discovery year
2009-08-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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