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Availability
Please contact us to check the availability
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Immunogen
Synthetic polypeptides from N-terminal domaine of p63
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Specificity
Human p40 protein, delta-Np63
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Species reactivity
Human
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Product presentation
Affinity purified antibody in PBS, BSA, sodium azide (0.05%)**. Use antibody dilution buffer (e.g. Art. No. PU002) containing sufficient protein and preservative.
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
IgG
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Antibody s clone
ZR8
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Product is supplied as
ready to use
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Recommended concentration for use
neat
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Protein concentration
N/A
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Positive control
Squamous Cell Carcinoma, Prostate, Skin
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Pretreatment
Pre-treatment of formaldehyde-fixed tissue with Tris-buffered EDTA solution pH 9 (Art.-No. DE006) or Unmasking Fluid G (Art.-No. DE007) 40 min at 100°C
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Tested applications
IHC(P)
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Recommended secondary reagent
VECTASTAIN Elite ABC Rabbit IgG (PK-6101) or ImmPRESS Rabbit IgG (MP-7401) or other polymer kits detecting rabbit IgG.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Guojun Wu, Shuji Nomoto, Mohammad Obaidul Hoque et al. (2003) ΔNp63α and TAp63α Regulate Transcription of Genes with Distinct Biological Functions in Cancer and Development Cancer Res May 15, 2003 63; 2351 2. Justin A Bishop, Julie Teruya-Feldstein, William H Westra, Giuseppe Pelosi, William D Travis and Natasha Rekhtman (2011) p40 (ΔNp63) is superior to p63 for the diagnosis of pulmonary squamous cell carcinoma. Modern Pathology (2012) 25, 405–415; doi:10.103 3. Verena Sailer, Carsten Stephan, Nicolas Wernert, Sven Perner, Klaus Jung, Manfred Dietel, Glen Kristiansen (2013) Comparison of p40 (ΔNp63) and p63 expression in prostate tissues – which one is the superior diagnostic marker for basal cells? Histopathology Volume 63, Issue 1, pages 50–56
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus