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Availability
Please contact us to check the availability
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Immunogen
Synthetic human p120 catenin protein
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Specificity
Catenin Deta 1; p120 Catenin
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Species reactivity
Human
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Product presentation
Affinity purified antibody in PBS containing 0.2%BSA and 0.09% sodium azide
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
IgG
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Antibody s clone
SP63
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Product is supplied as
liquid concentrate
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Recommended concentration for use
IHC (C,P) 1:50-1:200
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Protein concentration
N/A
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Positive control
Lobular carcinoma of the breast, Melanoma
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Pretreatment
Pretreatment of paraffin sections at high temperature is required. Suitable buffers are 0.01 M Citrate buffer (Art. No. DE000) or TRIS/EDTA buffer (pH 9; Art. No. DE006).
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Tested applications
IHC(C, P)
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Recommended secondary reagent
Anti-mouse IgG conjugate, biotinylated (Art. No.ZU102) in combination with streptavidin:HRPO (Art. No. ZU054)
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Storage method
Store at 2-8°C
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UniProt number
O6Go to UniProt/SwissProt716 (CTND1_HUMAN)
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Litterature
1. Reynolds AB, Herbert L, et al. p120, a novel substrate of protein tyrosine kinase receptors and of p60, is related to cadherin-binding factors β-catenin, plakoglobin, and armadillo. Oncogene 1992;7: 2439-2445. 2. Thoreson MA, Anastasiadis PZ, et al. Selective uncoupling of p120 from E-cadherin complexes. Mol Cell Biol. 1994;14: 8333-8342. 3. Sarrio D, Perez-Mies B, et al. Cytoplasmic localization of p120ctn and E-cadherin loss characterize lobular breast carcinoma from preinvasive to metastatic lesions. Oncogene. 2004 Apr 22;23(19):3272-83. 4. Dabbs DJ, Bhargava R, Chivukula M. The diagnostic utility of p120 catenin. Am J Surg. Pathol. 2007 31(3):427-37.
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Tips
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
