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Availability
Please contact us to check the availability
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Immunogen
A synthetic peptide from C-terminus of human CD117 / c-kit protein
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Specificity
Human CD117, c-kit Onkoprotein, SCFR (Stem Cell Factor Receptor) 145 kDa in WB
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Species reactivity
Human, Dog
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Product presentation
Cell culture supernatant containing sodium azide 0.09%.
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
IgG
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Antibody s clone
YR145
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Product is supplied as
ready to use
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Recommended concentration for use
neat
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Protein concentration
S/N
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Positive control
Skin, bone marrow, GIST
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Pretreatment
We recommend high temperature unmasking with Unmasking Fluid E (Tris-buffered EDTA solution pH 8.2, Art. No. DE006), which is advantageous to citrate buffer pre-treatment.
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Tested applications
IHC(C, P)
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
P1Go to UniProt/SwissProt721 - KIT_HUMAN
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Litterature
1. Natali P.G., Nicotra M.R., Winkler A.B., et al. (1992) Progression of human cutaneous melanoma is associated with loss of expression of c-kit proto-oncogene receptor. Int. J. Cancer 52; 197-201. 2. Matsuda R., Takahashi T., Nakamura S., et al. (1993) Expression of the c-kit protein in human solid tumors and in corresponding fetal and adult normal tissues. Am. J. Pathol. 142(1); 339-346. 3. Izquierdo M.A., Van der Valk P., Van Ark-Otte J. et al. (1995) Differential expression of the c-kit proto-oncogene in germ cell tumors. J. Pathol. 177; 253-258. 4. Miettinen M., and Lasota J. (2003) Gastrointestinal stromal tumors (GISTs): definition, occurence, pathology, differential diagnosis and molecular genetics. Pol J. Pathol. 54(1); 3-24.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
KIT
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Short name
Research
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Technique
Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Monoclonals or monoclonal antibodies
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Alternative name
CD117 (SCFR, c-kit Onkoprotein)
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Alternative technique
rabbit-anti, antibodies, kits, immunoglobulins
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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