Rabbit anti Rat IgG H + L rhodamine

  • Catalog number
    43R-1555
  • Price
    Please ask
  • Size
    1 mg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Purified Rat IgG (H&L)
  • Raised in
    Rabbit
  • Specificity
    This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.
  • Cross Reactivity
    No reactivity is observed to non-immunoglobulin rat serum proteins.
  • Conjugate Tag
    Rhodamine
  • Grade Purity
    Grater than 95% pure
  • Method of Purification
    Affinity purified using solid phase Rat IgG (H&L)
  • Source
    Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
  • Concentration
    1.0 mg/ml (lot specific)
  • Form Buffer
    Lyophilized from 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2. Dilute in dH2O to a concentration of 1 mg/ml
  • Storage
    Store at 2-8 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    FC; IF; IHC
  • Usage Recommendations
    The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Oryctolagus cuniculus, Rattus norvegicus
  • Gene target
  • Short name
    Rabbit anti IgG H + L rhodamine
  • Technique
    Rabbit, anti, IgG, antibody to, IgGs
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG, IgG
  • Label
    Rhodamine
  • Species
    Rat, Rats
  • Alternative name
    Rabbit anti Rat IgG (H + L) secondary antibody (rhodamine)
  • Alternative technique
    rabbit-anti, antibodies, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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