Rabbit Bone Morphogenetic Protein 4 (BMP4) CLIA Kit, 96 well plate

  • Catalog number
    EKU09943
  • Price
    Please ask
  • Size
    96T
  • Verified reactivity
    Oryctolagus cuniculus (Rabbit)
  • Detection limits
    15.62-1000 pg/mL
  • Sensitivity limit
    0,62 pg/mL
  • Samples to be analyzed
    Serum, plasma, tissue homogenates and other biological fluids
  • Assay duration
    2 hours, 40min
  • Verified applications
    CLIA
  • Assay class
    Sandwich
  • ELISA detection
    Chemiluminescent
  • Protein number
    O46576
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    Please refer to GenBank
  • Gene name
    Bone Morphogenetic Protein 4
  • Alternate gene name
    BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B
  • Estimated production time
    7-11 business days
  • Shipping requirements
    Blue ice
  • Storage recommendation
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    8 months
  • ELISA s specificity
    This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 4 (BMP4). No significant cross-reactivity or interference between Bone Morphogenetic Protein 4 (BMP4) and analogues was observed.
  • ELISA s cross reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 4 (BMP4) level in the sample or standard.;
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA s stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    Developmental science;Bone metabolism;
  • Warnings
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Description
    A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
  • About
    Rabbits are used for polyclonal antibody production by bioma. Rabbit antibodies are very stable and can be stored for several days at room temperature. bioma adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    Bone   Morphogenetic   Protein   BMP4   CLIA   Kit  
  • Gene symbol
    BMP4
  • Short name
    Rabbit Bone Morphogenetic Protein 4 (BMP4) CLIA Kit, 96
  • Technique
    Rabbit
  • Host
    Rabbit, Rabbits
  • Alternative name
    production species: rabbit Bone Morphogenetic Protein 4 (bone morphogenetic protein 4) CLIA reagent, 96 well plate
  • Alternative technique
    rabbit-anti, kits
  • Alternative to gene target
    bone morphogenetic protein 4, BMP2B and BMP2B1 and MCOPS6 and OFC11 and ZYME, BMP4 and IDBG-6966 and ENSG00000125378 and 652, BMP receptor binding, Extracellular, Bmp4 and IDBG-153687 and ENSMUSG00000021835 and 12159, BMP4 and IDBG-646706 and ENSBTAG00000003835 and 407216
  • Tissue
    bone
Gene info
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