Rabbit anti Sheep IgM (Fc specific), conjugated with Horseradish peroxidase, Secondary Antibodies
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Catalog number
RASh-IgM(Fc)-PO
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Price
Please ask
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Size
1ml
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Host Source
Rabbit
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Clone Name
Polyclonal
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Product Category
Veterinary
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Species Reactivity
Sheep
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Application
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
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Immunogen
Purified normal IgM isolated from pooled sheep serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
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Purification Method
NA
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Shipping Conditions
NA
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Storage conditions
Rabbit anti Sheep IgM (Fc specific), conjugated with Horseradish peroxidase, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years. Reconstituted at +4ÁC--7 days
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Datasheet link
NA
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
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About
Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Rabbit anti IgM (Fc specific), conjugated with Horseradish , Secondary Antibodies
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Technique
Rabbit, anti, IgM, FC, antibody to, antibody Conjugates, IgMs
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Host
Rabbit, Rabbits
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Isotype
NA, IgM
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Label
horseradish
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Species
Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
production species: rabbit antibody to ovine Immunoglobulin M (fragment c specific), coupled including Horseradish peroxidase, secondary detector Antibody Antibodies
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Alternative technique
rabbit-anti, antibodies, immunoglobulin-m
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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