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Immunogen
Synthetic peptide mapping to AA 809 to 831 of rat sequence
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Antibody s target
Rat NHE3
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Antibody s full description
Rabbit Anti-Rat NHE3 Polyclonal
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Primary research fields
Cell Signaling, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters, Cancer
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
SLC9A3 Antibody, Na+/H+ exchanger 3 Antibody, isoform 3 Antibody, Solute carrier family 9 member 3 Antibody, NHE3MGC126720 Antibody, MGC126718 Antibody, solute carrier family 9 (sodium/hydrogen exchanger) member 3 Antibody, NHE-3 Antibody, Na(+)/H(+) exchanger 3 Antibody, Sodium/hydrogen exchanger 3 Antibody
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Rat, Mouse
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~84kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
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Bibliography
1. Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942. 2. Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.
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Released date
11/Jun/2010
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NCBI number
NP_036786
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Gene number
24784
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Protein number
P26433
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PubMed number
27443990|26839400|26224717|25925252|23281477
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Tested applications
Western Blot|Western Blot||Western Blot|Western Blot
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Tested reactivity
Rat|Mouse||Mouse|Mouse
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400). Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400) at 1:1000. Rabbit Anti-NHE3 Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-400)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
