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Antibody category
Polyclonal Antibody
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Antibody s description
The polyclonal antibody was raised against collagen type IX from rat chondrosarcoma. It was purified from rabbit neat serum by affinity chromatography.
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Raised in
Rabbit
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Research area of interest
Diseases associated with collagen IX: EDM2 and EDM3
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Package form
liquid
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Clonality
Polyclonal
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Antigen
collagen type IX from rat chondrosarcoma
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Reacts with
rat, mouse, bovine, human
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Immunogen
See the data sheet or contact us
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Contents
PBS
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Purity
>95%
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Tested applications
• EIA • ELISA, • Flow Cytometry • Immunoblotting, suggested dilution 300 ng/ml - 1200 ng/ml • Immunofluorescence • Immunohistochemistry, suggest dilution 5 µg/ml - 15 µg/ml • Immunoprecipitation, suggest dilution 10 µg/ml - 30 µg/ml
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Other names
The polyclonal antibody was raised against collagen type IX from rat chondrosarcoma. It was purified from rabbit neat serum by affinity chromatography.
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Crossreactivity
no crossreactivty
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Antibody specificity
reacts with collagen type IX from rat, mouse, human and bovine. It exhibits no cross reactivity
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Purification
Protein A purified
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PubMed citations
See the data sheet
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UniProt number
N/A
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Storage conditions
- 70°C
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Technical datasheet
Contact us to receive the datasheet
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Shipping condition
dry ice
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Warnings
For Research Use only.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Gene
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by biotez. Rabbit antibodies are very stable and can be stored for several days at room temperature. biotez adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
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Gene target
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Gene symbol
COL9A3, COL9A1, COL9A2
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Short name
Rabbit anti- -Collagen IX (IgG), polyclonal
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Technique
Polyclonal, Rabbit, anti-, anti, IgG, antibody to, antibodies, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Label
not available conjugated
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Species
Rat, Rats
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Alternative name
production species: rabbit Antibody torat-Collagen classification IX (Immunoglobulin G), polyclonal
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Alternative technique
polyclonals, rabbit-anti, antibodies, immunoglobulins
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