Rabbit Anti-Rat Aquaporin 1 Polyclonal: APC

  • Catalog number
    SPC-502D-APC
  • Price
    Please ask
  • Size
    100 µg
  • Immunogen
    Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1
  • Antibody s target
    Rat Aquaporin 1
  • Antibody s full description
    Rabbit Anti-Rat Aquaporin 1 Polyclonal
  • Primary research fields
    Neuroscience, Ion Channels, Aquaporins
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    AQP1 Antibody, AQP 1 Antibody, AQP CHIP Antibody, AQP-1 Antibody, AQP1 Antibody, AQP1_HUMAN Antibody, Aquaporin CHIP Antibody, Aquaporin-1 Antibody, Aquaporin-CHIP Antibody, Aquaporin1 Antibody, Channel forming integral protein 28kDa Antibody, Channel like integral membrane protein 28 kDa Antibody, CHIP 28 Antibody, CHIP28 Antibody, CO Antibody, Colton blood group Antibody, Growth factor induced delayed early response protein Antibody, MGC26324 Antibody, Urine water channel Antibody, Water channel protein CHIP 29 Antibody, Water channel protein CHIP29 Antibody, Water channel protein for red blood cells and kidney proximal tubule Antibody
  • Verified applications
    WB, IHC, ICC/IF
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:2000), IHC (1:200), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Protein A Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~28.5kDa. May detect larger glycosylated bands ~35-50kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    0.5 µg/ml of SPC-502 was sufficient for detection of aquaporin 1 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Membrane
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin (3).
  • Bibliography
    1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. www.vivo.colostate.edu/hbooks/molecules/aquaporins.html
  • Released date
    1/Dec/2011
  • NCBI number
    NP_036910.1
  • Gene number
    25240
  • Protein number
    P29975
  • PubMed number
    27413198|27277500
  • Tested applications
    |Immunocytochemistry/Immunofluorescence
  • Tested reactivity
    |Mouse
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 1 protein using Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502). Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502) at 1:2000. Showing glycosylated and non-glycosylated bands. Rabbit Anti-Aquaporin 1 Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-502) | Rabbit Anti-Aquaporin 1 Antibody used in Western blot (WB) on Rat kidney inner medullary homogenates (SPC-502)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Oryctolagus cuniculus, Rattus norvegicus
  • Gene target
    Aquaporin   APC  
  • Gene symbol
    APC
  • Short name
    Rabbit Anti- Aquaporin 1 Polyclonal: APC
  • Technique
    Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    APC
  • Species
    Rat, Rats
  • Alternative name
    production species: rabbit Antibody toRat Aquaporin 1 polyclonal: adenomatous polyposis coli
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
  • Alternative to gene target
    adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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