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Category
Primary Antibodies
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Long description
This antibody recognizes Ribosomal Protein S6 Kinase with a molecular mass of 70 kDa (p70-S6K of p70-S6K 1). p70-S6K is a member of a family of serine/threonine protein kinases that phosphorylate the 40S ribosomal protein S6, thereby modulating the translation of ribosomal proteins and translation elongation factors. An isoform, p85-S6K, is derived from the same gene and is identical to p70-S6K with 23 extra residues at the N-terminus, which encode a nuclear localizing signal. p70-S6K is activated in response to the phosphatidylinositol 3-kinase (PI3-K) and mitogen-activated protein kinase (MAPK) pathways, and is required for progression through the cell cycle and for cell growth. Activation of p70-S6K is regulated by phosphorylation of seven different residues distributed throughout the protein, with the critical sites being threonines 229, 389, 421 and serine 424. Phosphorylation is progressive, culminating in phosphoregulation of threonine 229 within the activation loop. This antibody also recognizes p85-S6K._x000B_
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Antibody come from
n/a
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Other description
Purified IgG in phosphate buffered saline, pH 7.4. Protein A/G Chromatography
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti p70S6K Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human, Mouse, Rat
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, ELISA
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Antibody s suited for
The antibody has been used to detect A 43 in dot blots, RIA, ELISA and other related assays. For dot blot applications, we recommend using the antibody at 0.05-1.0 µg/mL. The optimal antibody concentration should be determined for each specific application.
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Storage
-20ºC
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Relevant references
1. Borchelt, D.R. et al. (1997) Accelerated amyloid deposition in the brains of transgenic mice coexpressing mutant presenilin 1 and amyloid precursor proteins. Neuron_x000B_19:939-945._x000B_2. Savage, M.J. et al. (1998) Turnover of amyloid -protein in mouse brain and acute reduction of its level by phorbol ester. J. Neurosci. 18:1743-1752._x000B_3. Cotman, C.W. (1997) The beta-amyloid peptide, peptide self-assembly, and the emergence of biological activities. A new principle in peptide function and the induction of neuropathology. Ann. N. Y. Acad. Sci. 814:1-16._x000B_4. Estus, S. et al. (1997) Aggregated amyloid-beta protein induces cortical neuronal apoptosis and concomitant apoptotic pattern of gene induction. J. Neurosci. 17:7736-7745._x000B_5. Bancher, C. et al. (1997) Mechanisms of cell death in Alzheimer's disease. J. Neural Transm. Suppl. 50:141-152._x000B_6. Russo, C. et al. (1998) Opposite roles of apolipoprotein E in normal brains and in Alzheimer's disease. Proc. NatÕl. Acad. Sci. USA 95:15598-15602.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus