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Category
Primary Antibodies
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Long description
Nuclear factor kappa B (NFkB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NFkB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF alpha LPS and mitogens. A serine/threonine protein kinase which mediates NFkappa;B activation by IL-1, TNF alpha; and CD95 was identified recently and designated NIK (for NF kappa;B inducing kinase) . NIK is an activator of I kappa B kinase alpha; and beta; (IKK alpha; and IKK beta;) (2-5). Therefore, NIK is a key molecule in the NF kappa;B signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
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Antibody come from
Rabbit polyclonal TLR2 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TLR2.
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Other description
Provided as solution in phosphate buffered saline with 0.02% sodium azide. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti NIK (CT) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
TLR2 (NT) antibody can be used for detection of TLR2 by Western blot at 0.5 to ug/ml. Despite its predicted molecular weight, TLR2 runs at ~100 kDa in SDS-PAGE. This polyclonal antibody can also detect TLR2 by immunohistochemistry at 2ug/ml.
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Storage
-20ºC
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Relevant references
1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. _x000B_2) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. _x000B_3.) Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. _x000B_4) OÕNeill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9. _x000B_5) McGettrick AF and OÕNeill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol Imm. 2004; 41:577-82. _x000B_6) Asehnoune K, Strassheim D, Mitra S, et al. Involvement of PKCalpha/beta in TLR4 and TLR2 dependent activation of NF-kappaB. Cell Signal. 2005; 17:385-94. _x000B_7) Wetzler LM. The role of Toll-like receptor 2 in microbial disease and immunity. Vaccine 2003; 21 Suppl 2:55-60. _x000B_8) UniProtKB/Swiss-Prot entry O60603, more Accessed February 8, 2007.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus