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Category
Primary Antibodies
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Long description
Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research and embryology, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.
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Antibody come from
The anti-GFP antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with highly purified native GFP derived from Aequorea victoria jellyfish.
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Other description
Each vial contains 100µl 1 mg/ml affinity purified polyclonal antiserum in 10 mM TRIS buffer pH 8.0, containing 0.02% sodium azide.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti-native Green Fluorescent Protein (Immunoaffinity purified) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
n/a
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Antibody s reacts with these species
Aequoria Victoria (native) GFP, recombinant (E.Coli) and native GFP-fusion proteins, and all variants of GFP tested.
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Antibody s specificity
The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.
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Application
Western Blotting, ELISA
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Antibody s suited for
The antibody is suitable for the detection of GFP by western blotting and ELISA. Optimal antibody dilutions for the different applications should be determined by titration. Recommended dilution for ELISA: 1:5,000 to !:25,000. For immunoblotting a dilution of 1:2,000 to 1:10,000 is recommended.
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Storage
Store at 4oC, or in small aliquots at -20oC.
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Relevant references
1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
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Protein number
UniProt: P42212 (A.victoria)
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
