-
Antibody category
Polyclonal Antibody
-
Antibody s description
The polyclonal antibody was raised against collagen type I from fetal mouse skin (FMS). The IgG fraction was purified from rabbit neat serum by affinity chromatography.
-
Raised in
Rabbit
-
Research area of interest
Diseases associated with collagen I: osteogenesis imperfecta, Ehlers-Danlos Syndrome, Infantile cortical hyperostosis aka Caffey's disease
-
Package form
liquid
-
Clonality
Polyclonal
-
Antigen
collagen type I from fetal mouse skin (FMS).
-
Reacts with
human, rat, mouse
-
Immunogen
See the data sheet or contact us
-
Contents
PBS
-
Purity
>95%
-
Tested applications
• EIA,• ELISA, • Flow Cytometry • Immunoblotting, suggested dilution 500ng/ml-1500 ng/ml • Immunofluorescence • Immunohistochemistry, suggested dilution 5 µg/ml-20 µg/ml • Immunoprecipitation, suggested dilution 15 µg/ml-45 µg/ml
-
Other names
The polyclonal antibody was raised against collagen type I from fetal mouse skin (FMS). The IgG fraction was purified from rabbit neat serum by affinity chromatography.
-
Crossreactivity
very slightly crossreactivity with collagen type III.
-
Antibody specificity
reacts with collagen type I from human, rat and mouse. It exhibits very slightly cross reactivity with collagen type III
-
Purification
Protein A purified
-
PubMed citations
See the data sheet
-
UniProt number
N/A
-
Storage conditions
- 70°C
-
Technical datasheet
Contact us to receive the datasheet
-
-
Shipping condition
dry ice
-
Warnings
For Research Use only.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Gene
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by biotez. Rabbit antibodies are very stable and can be stored for several days at room temperature. biotez adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Oryctolagus cuniculus
-
Group
Polyclonals and antibodies
-
Gene target
-
Gene symbol
COL1A2, COL1AR, COL1A1
-
Short name
Rabbit anti-Mouse Collagen I (IgG), polyclonal
-
Technique
Polyclonal, Rabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
IgG, IgG
-
Label
not available conjugated
-
Species
Mouse, Mouses
-
Alternative name
production species: rabbit Antibody toMouse Collagen classification I (Immunoglobulin G), polyclonal
-
Alternative technique
polyclonals, rabbit-anti, murine, antibodies, immunoglobulins
-