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Immunogen
Methylated KLH Conjugated
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Antibody s target
Methylated Lysine
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Antibody s full description
Rabbit Anti-Methylated Lysine Polyclonal
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Primary research fields
Cell Signaling, Post-Translational Modifications, Methylation
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Dimethylysine Antibody, Methyl lysine Antibody, N epsilon dimethyl lysine Antibody, Trimethyl lysine Antibody
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Verified applications
WB, ICC/IF, IP, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Species Independent
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Antibody s recommended dilutions for use
WB (1:5000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol
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Antibody s concentration
0.25 mg/ml
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Antibody s specificity
Detects proteins containing methylated lysine residues.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
0.2-0.5 µg/ml of SPC-158 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.
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Antibody in cell
Contact our support service.
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
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Bibliography
1. Yang X.J. (2005) Oncogene. 24:1653-1662. 2. Hassig C.A. and Schreiber S.L. (1997) Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang X.J. (2004) Bioessays 26:1076-1087. 4. Hughes R.E. (2002) Curr. Biol. 12: R141-R143. 5. Vigushin D.M. and Coombes R.C. (2004) Curr. Cancer Drug Targets 4: 205-218. 6. Chan H.M. et al. (2001) Nat. Cell Biol. 3: 667-674. 7. Martinez-Balbas M.A. et al. (2000) EMBO J. 19: 662-671.
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Released date
1/Jun/2007
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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PubMed number
27858264|27521690|25609799|25374779|24651940|24233354|23533717
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Tested applications
Dot Blot|Western Blot|Western Blot|Western Blot|Dot Blot|Dot Blot|Dot Blot
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Tested reactivity
Otala lactea (milk snail)|Squirrel|Hamster |Ictidomys tridecemlineatus (ground squirrel)|Xenopus laevis|Littorinalittorea (intertidal marine snail)|Turtle
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Methylated Lysine Antibody. (C) Composite. | Western blot analysis of Bovine serum albumin showing detection of Methylated Lysine protein using Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158). Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody (SPC-158) at 1:1000. Methylated Lysine in BSA (Left) and Methylated BSA (Right). Rabbit Anti-Methylated Lysine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-158) | Rabbit Anti-Methylated Lysine Antibody used in Western blot (WB) on Bovine serum albumin (SPC-158)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.4
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
