Rabbit anti MAPKKK5 (CT) antibody (ASK 1(CT)

• Catalog number
  X1124P
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF- -induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
• Antibody come from
  Synthetic peptide corresponding to amino acids 1356 to 1375 of human ASK1 protein. Immunogen sequence differs in mouse by last two amino acids.
• Other description
  Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Rabbit anti MAPKKK5 (CT) antibody (ASK 1(CT) Antibody
• Antibody is raised in
  Rabbit
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot
• Antibody s suited for
  Detects ASK1 by Western blot at 0.5 to 1 µg/ml. Detects a 155 kDa band in SW1353 whole cell lysate. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -20ºC
• Relevant references
  1. Ichijo, H., et al., 'Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways.' Science 1997, 275, 90-94 _x000B__x000B_2. Wang, X.S., et al., 'Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase.' J. Biol. Chem. 1996, 271, 31607-31611 _x000B__x000B_3. Tobiume, K., et al., 'Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1.' Biochem. Biophys. Res. Commun. 1997, 239, 905-910 _x000B_
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  MAPKKK5   ASK  
• Gene symbol
  MAP3K5, DBF4
• Short name
  Rabbit anti MAPKKK5 (CT) antibody (ASK 1(CT)
• Technique
  Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for
• Host
  Rabbit, Rabbits
• Isotype
  not specified
• Label
  unlabeled
• Alternative name
  production species: rabbit antibody to MAPKKK5 (CT) (antibody to-) (ASK 1(CT)
• Alternative technique
  antibodies, rabbit-anti

Gene info

• Identity
  HGNC:6857
• Gene
  MAP3K5
• Long gene name
  mitogen-activated protein kinase kinase kinase 5
• Synonyms gene
  • MEKK5
• Synonyms
  • MAPKKK5
  • ASK1
• Synonyms name
  • apoptosis signal regulating kinase 1
• GenBank acession
  • U67156
• Locus
  6q23.3
• Discovery year
  1997-11-14
• Entrez gene record
  4217
• Pubmed identfication
  • 9465908
• Classification
  • Mitogen-activated protein kinase kinase kinases
• VEGA ID
  OTTHUMG00000015647
• Locus Specific Databases
  • The Globin Gene Server

Gene info

• Identity
  HGNC:17364
• Gene
  DBF4
• Long gene name
  DBF4 zinc finger
• Synonyms gene name
  • DBF4 homolog (S. cerevisiae)
• Synonyms
  • ASK
  • chif
  • ZDBF1
  • DBF4A
• Synonyms name
  • activator of S phase kinase
  • chiffon homolog (Drosophila)
  • zinc finger, DBF-type containing 1
  • DBF4 zinc finger A
• GenBank acession
  • AF160876
• Locus
  7q21.12
• Discovery year
  2005-11-15
• Entrez gene record
  10926
• Pubmed identfication
  • 10373557
  • 10517317
• RefSeq identity
  • NM_006716
• Classification
  • Zinc fingers DBF-type
• VEGA ID
  OTTHUMG00000131034

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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