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Immunogen
KDEL containing peptide immunogen
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Antibody s target
KDEL
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Antibody s full description
Rabbit Anti-KDEL Polyclonal
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Primary research fields
Cell Signaling, Organelle Markers, Trafficking, Chaperones
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Lys Asp Glu Leu Antibody
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Verified applications
WB, ICC/IF, IHC
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS pH7.2, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.
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Antibody in cell
Endoplasmic Reticulum
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
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Bibliography
1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610. 2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.
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Released date
16/May/2011
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody (SPC-109). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody (SPC-109) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. | Western blot analysis of Human Cell line lysates showing detection of KDEL protein using Rabbit Anti-KDEL Polyclonal Antibody (SPC-109). Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody (SPC-109) at 1:1000, 1:500. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-KDEL Polyclonal Antibody (SPC-109). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-KDEL Polyclonal Antibody (SPC-109) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. Rabbit Anti-KDEL Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-109) | Rabbit Anti-KDEL Antibody used in Western blot (WB) on Human Cell line lysates (SPC-109) | Rabbit Anti-KDEL Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-109)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.2
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
