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Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr60 of human WNK1 (AA57-63)
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Antibody s target
Human WNK1 (pThr60)
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Antibody s full description
Rabbit Anti-Human WNK1 (pThr60) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Erythrocyte 65 kDa protein Antibody, HSAN2 Antibody, HSN2 Antibody, hWNK1 Antibody, KDP Antibody, KIAA0344 Antibody, Kinase deficient protein Antibody, MGC163339 Antibody, MGC163341 Antibody, p65 Antibody, PRKWNK1 Antibody, Prostate derived sterile 20 like kinase Antibody, Protein kinase lysine deficient 1 Antibody, Protein kinase lysine-deficient 1 Antibody, Protein kinase with no lysine 1 Antibody, PSK Antibody, Serine/threonine protein kinase WNK1 Antibody, Serine/threonine-protein kinase WNK1 Antibody, With no K Antibody, WNK lysine deficient protein kinase 1 Antibody, WNK1 Antibody, WNK1_HUMAN Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 250.756 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1098 was sufficient for detection of WNK1 (pThr60) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
Highly expressed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
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Scientific context
WINK1 is a serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signalling, survival, and proliferation.WNK1 activates SCNN1A, SCNN1B, SCNN1D and SGK1. Exact mechanism of oncogenesis is unclear.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_061852
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Gene number
65125
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Protein number
Q9H4A3
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
WNK1
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Short name
Rabbit Anti- WNK1 (pThr60) Polyclonal: ATTO 655
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 655
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman WNK lysine deficient protein kinase 1 (pThr60) polyclonal: ATTO 655
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
WNK lysine deficient protein kinase 1, WNK1 and IDBG-11521 and ENSG00000060237 and 65125, transferase activity, Cytoplasm, Wnk1 and IDBG-183036 and ENSMUSG00000045962 and 232341, WNK1 and IDBG-641017 and ENSBTAG00000005221 and 506433
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