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Immunogen
Synthetic peptide from the C-terminal of human UVRAG
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Antibody s target
Human UVRAG
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Antibody s full description
Rabbit Anti-Human UVRAG Polyclonal
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Primary research fields
Cancer, Apoptosis, Autophagy, Cell Signaling, Epigenetics, Cardiovascular System, Heart
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
DHTX Antibody, p63 Antibody, UV radiation resistance associated Antibody, UVRAG_HUMAN Antibody
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Predicted molecular weight at ~78.1kDa. Observed molecular weights in the 75-90kDa range.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-605 was sufficient for detection of UVRAG on 293T Rapamycin treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Endosome, Lysosome, Endoplasmic reticulum, Nucleus, Chromosome, Centromere
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (1). Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (2). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (3-4). It is highly expressed in the brain, lung, kidney and liver.
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Bibliography
1. Liang, C. et al. (2008) Nat Cell Biol. 10: 776-87. 2. Ionov, Y. et al. (2004) Oncogene. 23: 639-45. 3. Kim, M.S. et al. (2008) Hum Pathol. 39: 1059-63.
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Released date
1/Oct/2015
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NCBI number
NP_003360.2
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Gene number
7405
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Protein number
Q9P2Y5
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) UVRAG Antibody (D) Composite. | Western blot analysis of Human 293T showing detection of ~70kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605). Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~70kDa. | Western blot analysis of Rat Liver showing detection of ~70kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~70kDa. Rabbit Anti-UVRAG Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-605) | Rabbit Anti-UVRAG Antibody used in Western blot (WB) on 293T (SPC-605) | Rabbit Anti-UVRAG Antibody used in Western blot (WB) on Liver (SPC-605)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
