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Immunogen
Peptide sequence around aa.18~22(S-N-S-E-E) derived from Human Twist1
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Antibody s target
Human Twist
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Antibody s full description
Rabbit Anti-Human Twist Polyclonal
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Primary research fields
Neuroscience, Cell Signaling, Epigenetics, Cancer, Cardiovascular System, Heart
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ACS3 Antibody, bHLHa38 Antibody, SCS Antibody, TWIST Antibody, Twist basic helix loop helix transcription factor 1 Antibody, Twist homolog 1 Antibody, Twist related protein 1 Antibody, TWIST1 Antibody
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Verified applications
WB, IHC
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~21kDa, endogenous levels of total Twist1 protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1336 was sufficient for detection of Twist in 10 µg of mouse muscle cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
Subset of mesodermal cells.
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Scientific context
TWIST1 is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. It has been implicated in cell lineage determination and differentiation. The strongest expression of this mRNA is in placental tissue, in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
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Bibliography
1. Reinhold M.I., Kapadia R.M., Liao Z., Naski M.S. (2006) J Biol Chem. 281(3): 1381-1388.
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Released date
6/Sep/2016
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NCBI number
NP_000465.1
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Gene number
7291
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Protein number
Q15672
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus