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Immunogen
Synthesized peptide derived from internal of human TNFA.
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Antibody s target
Human TNF alpha
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Antibody s full description
Rabbit Anti-Human TNF alpha Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
APC1 Antibody, APC1 protein Antibody, Cachectin Antibody, DIF Antibody, Differentiation inducing factor Antibody, Macrophage cytotoxic factor Antibody, TNF alpha Antibody, Tnf Antibody, TNF superfamily, member 2 Antibody, TNF, macrophage derived Antibody, TNF, monocyte derived Antibody, TNF-a Antibody, TNF-alpha Antibody, TNFA Antibody, TNFA_HUMAN Antibody, TNFSF2 Antibody, Tumor necrosis factor alpha Antibody, Tumor necrosis factor Antibody, Tumor necrosis factor ligand superfamily member 2 Antibody, Tumor Necrosis Factor, Membrane Form Antibody, Tumor necrosis factor, soluble form Antibody
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Verified applications
IHC, ICC/IF, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
IHC (1:100), ICC/IF (1:500), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects endogenous levels of total TNFA protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:100 dilution of SPC-1315 was sufficient for detection of TNF alpha in human HepG2 cells using Goat Anti-Rabbit IgG as the secondary antibody.
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Antibody in cell
Cell Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Tumor necrosis factor is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. The primary role of TNF is in the regulation of immune cells. TNF, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD).
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Bibliography
1. Swardfager W et al. (2010) Biol Psychiatry 68 (10): 930–941. 2. Locksley RM et al. (2001) Cell 104 (4): 487–501. 3. Dowlati Y et al. (2010) Biol Psychiatry 67 (5): 446–457. 4. Victor FC and Gottlieb AB (2002) J Drugs Dermatol 1 (3): 264–75. 5. Brynskov J. (2002) Gut 51 (1): 37–43.
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Released date
6/Sep/2016
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NCBI number
NP_000585.2
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Gene number
7124
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Protein number
P01375
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315). Tissue: Colon Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-TNF alpha Polyclonal Antibody (SPC-1315) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-TNF alpha Antibody used in Immunohistochemistry (IHC) on Colon Carcinoma Tissue (SPC-1315) | Rabbit Anti-TNF alpha Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on HepG2 cells (SPC-1315)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
The Rabbit Anti- TNF alpha Polyclonal: Alkaline Phosphatase is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
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Gene target
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Gene symbol
TNF, TNFRSF1A, TNFRSF1B, LTBR
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Short name
Rabbit Anti- TNF alpha Polyclonal: Alkaline Phosphatase
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
Alkaline Phosphatase
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman tumor necrosis factor a polyclonal: Alkaline Phosphatase
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
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