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Immunogen
Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
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Antibody s target
Human TLR4
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Antibody s full description
Rabbit Anti-Human TLR4 Polyclonal
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Primary research fields
Cell Signaling, Cardiovascular System, Atherosclerosis
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ARMD10 Antibody, CD284 Antibody, TLR 4 Antibody, TOLL Antibody, Toll like receptor 4 Antibody
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Verified applications
WB, IHC, ICC/IF, FCM, FACS
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:500), IHC (1:50); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).
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Bibliography
1. Muzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101. 2. Medzhitov R. and Janeway C.A (1997) Cell. 91: 295-298. 3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta. 1518(1-2): 157-161. 4. Takeuchi O., et al. (1999) Immunity. 11: 443. 5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA. 97: 2163-2167. 6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature. 388(6640): 394-397.
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Released date
1/Jan/2011
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NCBI number
NP_612564.1
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Gene number
7099
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Protein number
O00206
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PubMed number
26235132|25595264
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Tested applications
Western Blot|Immunohistochemistry
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Tested reactivity
Mouse|Mouse
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells. | Western blot analysis of Human, Mouse Cell line lysates showing detection of TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:1000. | Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: Spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100. | Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100. Rabbit Anti-TLR4 Antibody used in Immunohistochemistry (IHC) on Mouse colon colitis (SPC-200) | Rabbit Anti-TLR4 Antibody used in Western blot (WB) on Human, Mouse Cell line lysates (SPC-200) | Rabbit Anti-TLR4 Antibody used in Immunohistochemistry (IHC) on Mouse Spleen tissue (SPC-200) | Rabbit Anti-TLR4 Antibody used in Immunohistochemistry (IHC) on Mouse spleen tissue (SPC-200)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
TLR4
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Short name
Rabbit Anti- TLR4 Polyclonal: FITC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
FITC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman toll-like receptor 4 polyclonal: fluorecein
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Alternative technique
polyclonals, rabbit-anti, antibodies, fluorescine
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Alternative to gene target
toll-like receptor 4, ARMD10 and CD284 and TLR-4 and TOLL, TLR4 and IDBG-82738 and ENSG00000136869 and 7099, protein binding, Cell surfaces, Tlr4 and IDBG-156729 and ENSMUSG00000039005 and 21898, TLR4 and IDBG-638758 and ENSBTAG00000006240 and 281536
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