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Immunogen
Synthesized peptide derived from human Thyroid Hormone Receptor β antibody.
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Antibody s target
Human THRB
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Antibody s full description
Rabbit Anti-Human THRB Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2 Antibody, C ERBA 2 Antibody, C ERBA BETA Antibody, c-erbA-2 Antibody, c-erbA-beta Antibody, ERBA 2 Antibody, ERBA BETA Antibody, ERBA2 Antibody, Erythroblastic leukemia viral (v erb a) oncogene homolog 2 avian Antibody, generalized resistance to thyroid hormone Antibody, GRTH Antibody, MGC126109 Antibody, MGC126110 Antibody, NR1A2 Antibody, Nuclear receptor subfamily 1 group A member 2 Antibody, Oncogene ERBA2 Antibody, PRTH Antibody, THB_HUMAN Antibody, THR1 Antibody, THRB 1 Antibody, THRB 2 Antibody, thrB Antibody, THRB1 Antibody, THRB2 Antibody, Thyroid hormone nuclear receptor beta variant 1 Antibody, Thyroid hormone receptor beta 1 Antibody, Thyroid hormone receptor beta 2 Antibody, Thyroid hormone receptor beta Antibody, Thyroid hormone receptor, beta (erythroblastic leukemia viral (v erb a) oncogene homolog 2, avian) Antibody
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Verified applications
WB, IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~42kDa, endogenous levels of total Thyroid Hormone Receptor β protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1314 was sufficient for detection of THRB in 10 µg of LOVO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
There are three forms of the thyroid hormone receptor designated alpha-1, beta-1 and beta-2 that are able to bind thyroid hormone. There are two TR-α receptor splice variants encoded by the THRA gene and two TR-β isoform splice variants encoded by the THRB gene. Thyroid hormone receptor β-1 operates as a hormone-dependent transcriptional regulator that binds DNA sequences and is activated by 3,5,3’-l-triiodothyronine (T3). Thyroid hormone receptor β-1 is important in vertebrate development, specifically in developing limbs and face. 9-cis-retinoic acid receptor (RXR) and retinoic acid receptor (RAR) receptor families are often associated with thyroid hormone receptor β-1 and form homodimers and heterodimers.
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Bibliography
1. Flamant F et al. (2006) Pharmacol Rev 58 (4): 705–11.
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Released date
6/Sep/2016
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NCBI number
NP_000452.2
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Gene number
7068
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Protein number
P10828
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-THRB Polyclonal Antibody (SPC-1314). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-THRB Polyclonal Antibody (SPC-1314) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human LOVO cell lysates showing detection of ~42kDa THRB protein using Rabbit Anti-THRB Polyclonal Antibody (SPC-1314). Lane 1: Human LOVO cells. Lane 2: Human LOVO cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-THRB Polyclonal Antibody (SPC-1314) at 1:1000. Predicted/Observed Size: ~42kDa. Other Band(s): ~55kDa. Rabbit Anti-THRB Antibody used in Immunohistochemistry (IHC) on Lung Carcinoma Tissue (SPC-1314) | Rabbit Anti-THRB Antibody used in Western blot (WB) on LOVO cell lysates (SPC-1314)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
THRB-AS2, THRB-IT1, THRB-AS1, THRB
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Short name
Rabbit Anti- THRB Polyclonal: Streptavidin
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
Streptavidin
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman thyroid hormone receptor, b polyclonal: biotinilated-binding streptavidin
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
thyroid hormone receptor, beta, C-ERBA-2 and C-ERBA-BETA and ERBA2 and GRTH and NR1A2 and PRTH and THR1 and THRB1 and THRB2, THRB and IDBG-22332 and ENSG00000151090 and 7068, thyroid hormone binding, nuclei, Thrb and IDBG-133569 and ENSMUSG00000021779 and 21834, THRB and IDBG-630420 and ENSBTAG00000017802 and 788329
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