Rabbit Anti-Human STAT3 Polyclonal: Unconjugated

  • Catalog number
    SPC-1334D
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    A synthesized peptide derived from human STAT3
  • Antibody s target
    Human STAT3
  • Antibody s full description
    Rabbit Anti-Human STAT3 Polyclonal
  • Primary research fields
    Cell Signaling, Epigenetics, Cancer, Cardiovascular System, Heart
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    Acute Phase Response Factor Antibody, ADMIO Antibody, APRF Antibody, DNA binding protein APRF Antibody, HIES Antibody, Signal transducer and activator of transcription 3 Antibody, STAT 3 Antibody
  • Verified applications
    WB, IHC
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects ~85kDa, endogenous levels of total STAT3
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-1334 was sufficient for detection of STAT3 in 10 µg of K562 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Nucleus
  • Tissue specificity
    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Scientific context
    Stat3 is an important transciption factor for many cytokines and growth factor receptors. It is constitutively activated in a number of human tumors and has oncogenic potential and anti-apoptotic activites.
  • Bibliography
    1. Darnell J.E., et al. (1994) Science. 264: 1415-1421. 2. Garcia R. and Jove R. (1998) J Biomed Sci. 5: 79-85. 3. Bromberg J.F., et al. (1999) Cell. 98: 295-303.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_644805.1
  • Gene number
    6774
  • Protein number
    P40763
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure legend
    Western blot analysis of Human K562 cell lysates showing detection of ~86kDa STAT3 protein using Rabbit Anti-STAT3 Polyclonal Antibody (SPC-1334). Lane 1: Human K562 whole cell lysates treated with the immunizing peptide. Lane 2: Human K562 whole cell lysates. Primary Antibody: Rabbit Anti-STAT3 Polyclonal Antibody (SPC-1334) at 1:1000. Predicted/Observed Size: ~86kDa. Rabbit Anti-STAT3 Antibody used in Western blot (WB) on K562 cell lysates (SPC-1334)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Conjugation
    not conjugated 
  • Gene target
  • Gene symbol
    STAT3
  • Short name
    Rabbit Anti- STAT3 Polyclonal: Unconjugated
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG
  • Label
    Unconjugated
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman signal transducer and activator on transcription 3 (acute-phase response factor) polyclonal: nonconjugated
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
  • Alternative to gene target
    signal transducer and activator of transcription 3 (acute-phase response factor), ADMIO and APRF and HIES, STAT3 and IDBG-50702 and ENSG00000168610 and 6774, protein dimerization activity, nuclei, Stat3 and IDBG-211637 and ENSMUSG00000004040 and 20848, STAT3 and IDBG-640197 and ENSBTAG00000021523 and 101910056,508541
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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