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Immunogen
A synthesized peptide derived from human STAT3
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Antibody s target
Human STAT3
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Antibody s full description
Rabbit Anti-Human STAT3 Polyclonal
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Primary research fields
Cell Signaling, Epigenetics, Cancer, Cardiovascular System, Heart
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Acute Phase Response Factor Antibody, ADMIO Antibody, APRF Antibody, DNA binding protein APRF Antibody, HIES Antibody, Signal transducer and activator of transcription 3 Antibody, STAT 3 Antibody
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Verified applications
WB, IHC
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~85kDa, endogenous levels of total STAT3
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1334 was sufficient for detection of STAT3 in 10 µg of K562 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
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Scientific context
Stat3 is an important transciption factor for many cytokines and growth factor receptors. It is constitutively activated in a number of human tumors and has oncogenic potential and anti-apoptotic activites.
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Bibliography
1. Darnell J.E., et al. (1994) Science. 264: 1415-1421. 2. Garcia R. and Jove R. (1998) J Biomed Sci. 5: 79-85. 3. Bromberg J.F., et al. (1999) Cell. 98: 295-303.
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Released date
6/Sep/2016
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NCBI number
NP_644805.1
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Gene number
6774
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Protein number
P40763
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human K562 cell lysates showing detection of ~86kDa STAT3 protein using Rabbit Anti-STAT3 Polyclonal Antibody (SPC-1334). Lane 1: Human K562 whole cell lysates treated with the immunizing peptide. Lane 2: Human K562 whole cell lysates. Primary Antibody: Rabbit Anti-STAT3 Polyclonal Antibody (SPC-1334) at 1:1000. Predicted/Observed Size: ~86kDa. Rabbit Anti-STAT3 Antibody used in Western blot (WB) on K562 cell lysates (SPC-1334)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
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Gene target
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Gene symbol
STAT3
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Short name
Rabbit Anti- STAT3 Polyclonal: Unconjugated
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
Unconjugated
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman signal transducer and activator on transcription 3 (acute-phase response factor) polyclonal: nonconjugated
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
signal transducer and activator of transcription 3 (acute-phase response factor), ADMIO and APRF and HIES, STAT3 and IDBG-50702 and ENSG00000168610 and 6774, protein dimerization activity, nuclei, Stat3 and IDBG-211637 and ENSMUSG00000004040 and 20848, STAT3 and IDBG-640197 and ENSBTAG00000021523 and 101910056,508541
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