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Immunogen
N-terminal region of SOD1, exposed dimer interface (EDI)
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Antibody s target
Human SOD1
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Antibody s full description
Rabbit Anti-Human SOD1 (EDI) Polyclonal
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Primary research fields
Cancer, Oxidative Stress, Neuroscience, Neurodegeneration, ALS Disease
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
SEDI Antibody, SOD1 EDI Antibody, Superoxide dismutase 1 Antibody, SOD Antibody, SOD1 exposed dimer interface Antibody
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Verified applications
WB, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Recognizes a conformation specific epitope where the dimer interface is exposed.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1µg/ml of SPC-206 was sufficient for detection of the exposed dimer interface of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as SOD1 (EDI), which targets an exposed region of the dimer interface (EDI) of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with SOD1 (UβB) (SPC-205D) which detects an unfolded beta barrel (UβB) of SOD1.
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Bibliography
1. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 2. Furukawa Y., and O’Halloran T. (2006) Antioxid Redox Signal. 8(5-6):847-67. 3. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol. 284:L917-L925. 4. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385. 5. Kerman A., et al. (2010) Acta Neuropathol. 119:335-344.
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Released date
25/Sep/2013
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NCBI number
CAG46542
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Gene number
6647
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Protein number
P00441
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PubMed number
28057013
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Tested applications
ELISA
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Tested reactivity
Human
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Mouse Lung showing detection of ~18 kDa SOD1 (EDI) protein using Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Lung. Load: 20 µg. Primary Antibody: Rabbit Anti-SOD1 (EDI) Polyclonal Antibody (SPC-206) at 1:1000. Predicted/Observed Size: ~18 kDa. Rabbit Anti-SOD1 (EDI) Antibody used in Western blot (WB) on Mouse Lung (SPC-206)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
SOD1-DT, SOD1
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Short name
Rabbit Anti- SOD1 (EDI) Polyclonal: Streptavidin
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
Streptavidin
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman superoxide dismutase 1, soluble (EDI) polyclonal: biotinilated-binding streptavidin
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
superoxide dismutase 1, soluble, ALS and ALS1 and HEL-S-44 and homodimer and hSod1 and IPOA and SOD, SOD1 and IDBG-1609 and ENSG00000142168 and 6647, chaperone binding, nuclei, Sod1 and IDBG-174662 and ENSMUSG00000022982 and 20655
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