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Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr182 of human SIK1 (AA179-185)
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Antibody s target
Human SIK1 (pThr182)
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Antibody s full description
Rabbit Anti-Human SIK1 (pThr182) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
SNF1LK Antibody, KID2 Antibody, MSK Antibody, myocardial SNF1 like kinase Antibody, QIK Antibody, Qin-induced kinase Antibody, Salt inducible kinase 1 Antibody, Salt-inducible protein kinase 1 Antibody, Serine threonine protein kinase SNF1 like kinase 1 Antibody, Serine threonine protein kinase SNF1LK Antibody, Serine/threonine-protein kinase SIK1 Antibody, Serine/threonine-protein kinase SNF1-like kinase 1 Antibody, Serine/threonine-protein kinase SNF1LK Antibody, SIK-1 Antibody, SIK1 Antibody, SIK1_HUMAN Antibody, SNF1 like kinase Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 84.902 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1082 was sufficient for detection of SIK1 (pThr182) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
Highly expressed in the cornea, keratinocytes, and B-lympocytes. Lower expression found in many organs.
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Scientific context
SIK1 (salt-inducible kinase 1), also known as SNF1LK or MSK, is a 783 amino acid protein that contains one UBA domain and one protein kinase domain and belongs to the Ser/Thr protein kinase family. Localized to both the nucleus and the cytoplasm, SIK1 uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation. Additionally, SIK1 has a potential role in regulation of the G2/M cell cycle transition, as well as in inhibitory control of CREB protein function.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_775490
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Gene number
102724428
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Protein number
P57059
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
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Gene target
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Gene symbol
SIK1
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Short name
Rabbit Anti- SIK1 (pThr182) Polyclonal: Unconjugated
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
Unconjugated
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman salt-inducible kinase 1 (pThr182) polyclonal: nonconjugated
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
salt-inducible kinase 1, SIK1 and IDBG-4715 and ENSG00000142178 and 150094, transferase activity, nuclei, Sik1 and IDBG-164811 and ENSMUSG00000024042 and 17691, SIK1 and IDBG-639805 and ENSBTAG00000011437 and 100337254
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