-
Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr323 of human Syk (AA320-326)
-
Antibody s target
Human Syk (pTyr323)
-
Antibody s full description
Rabbit Anti-Human Syk (pTyr323) Polyclonal
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
p72-Syk Antibody, p72syk Antibody, Spleen tyrosine kinase Antibody, Syk Antibody, Tyrosine protein kinase SYK Antibody, Tyrosine-protein kinase SYK Antibody, EC 2.7.10.2 Antibody, kinase Syk Antibody, KSYK Antibody, KSYK_HUMAN Antibody
-
Verified applications
WB, AM
-
Raised in
Rabbit
-
Antibody s reactivity
Human
-
Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects 72.066 kDa.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:250 dilution of SPC-1085 was sufficient for detection of Syk (pTyr323) in 10 µg of mouse spleen lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Cell Membrane, Cytoplasm
-
Tissue specificity
Highly expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is expressed in pro-B-cells to plasma cells.
-
Scientific context
Syk is involved in BCR signal transduction. It can signal through PI3K or PLC depending on cell context and phoshrorylation state. SYK exprssion is lost in breast cancer and facilitate tumour growth and metastasis. A tranlocation with ETV6 mediates an overactive protein and dysregulated signal
-
Bibliography
Refer to PubMed
-
Released date
29/Aug/2016
-
NCBI number
NP_003168
-
Gene number
6850
-
Protein number
P43405
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
Representative figure link
No Data Available
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Short name
Rabbit Anti- Syk (pTyr323) Polyclonal: Streptavidin
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
Immunoglobulin
-
Label
Streptavidin
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman spleen tyrosine kinase (pTyr323) polyclonal: biotinilated-binding streptavidin
-
Alternative technique
polyclonals, rabbit-anti, antibodies
-
Alternative to gene target
spleen tyrosine kinase, p72-Syk, SYK and IDBG-75571 and ENSG00000165025 and 6850, transferase activity, Plasma membranes, Syk and IDBG-152695 and ENSMUSG00000021457 and 20963, SYK and IDBG-637026 and ENSBTAG00000004767 and 515515
-
