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Immunogen
C-terminal amino acids of human PUMA
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Antibody s target
Human PUMA
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Antibody s full description
Rabbit Anti-Human PUMA (CT) Polyclonal
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Primary research fields
Cancer, Apoptosis
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 0.02% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
1 µg/ml of SPC-166 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Mitochondrion
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Bibliography
1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94. 2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82. 3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.
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Released date
1/Jun/2007
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NCBI number
NP_001120712.1
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Gene number
27116
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Protein number
Q9BXH4
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166) at 1:125 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Human, Mouse K562 and 3T3 cell lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (SPC-166). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-166) at 1:1000. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody (SPC-166) at 1:125 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-166). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-166) at 1:100. Rabbit Anti-PUMA (CT) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-166) | Rabbit Anti-PUMA Antibody used in Western blot (WB) on Human, Mouse K562 and 3T3 cell lysates (SPC-166) | Rabbit Anti-PUMA (CT) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-166) | Rabbit Anti-PUMA Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human K562 cells (SPC-166)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
