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Immunogen
A synthesized peptide derived from human PTEN
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Antibody s target
Human PTEN
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Antibody s full description
Rabbit Anti-Human PTEN Polyclonal
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Primary research fields
Cell Signaling, Epigenetics, Cancer
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
GLM2 Antibody, MHAM Antibody, MMAC1 Antibody, Phosphatase and tensin like protein Antibody, Pten Antibody, PTEN1 Antibody, TEP1 Antibody
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Verified applications
WB, IHC
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~47kDa, endogenous levels of total PTEN
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1331 was sufficient for detection of PTEN in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
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Scientific context
PTEN, also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers. It is a major negative regulator of the PI3K/Akt signaling pathway. It also regulates p53 protein levels and is involved in G protein-coupled signaling during chemotaxis.
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Bibliography
1. Wan X. and Helman L.J. (2003) Oncogene. 22: 8205–8211. 2. Cantley L.C. and Neel B.G. (1999) Proc Natl Acad Sci USA. 96: 4240-4245. 3. Myers M.P., et al. (1998) Proc Natl Acad Sci USA. 95: 13513-13518. 4. Wu X., et al. (1998) Proc Natl Acad Sci USA. 95: 15587-15591. 5. Funamoto S., et al. (2002) Cell. 109: 611-623. 6. Iijima M. and Devreotes P. (2002) Cell. 109: 599-610.
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Released date
6/Sep/2016
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NCBI number
NP_000305.3
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Gene number
5728
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Protein number
P60484
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Human HeLa cell lysates showing detection of ~54kDa PTEN protein using Rabbit Anti-PTEN Polyclonal Antibody (SPC-1331). Lane 1: Vanadate treated Human HeLa whole cell lysates treated with the immunizing peptide. Lane 2: Vanadate treated Human HeLa whole cell lysates. Primary Antibody: Rabbit Anti-PTEN Polyclonal Antibody (SPC-1331) at 1:1000. Predicted/Observed Size: ~54kDa. Rabbit Anti-PTEN Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1331)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
PTEN, PTENP1
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Short name
Rabbit Anti- PTEN Polyclonal:
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
Biotin
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman phosphatase and tensin homolog polyclonal: biotinilated
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
phosphatase and tensin homolog, 10q23del and BZS and CWS1 and DEC and GLM2 and MHAM and MMAC1 and PTEN1 and TEP1, PTEN and IDBG-81409 and ENSG00000171862 and 5728, phosphatidylinositol-3, nuclei, Pten and IDBG-158878 and ENSMUSG00000013663 and 19211, PTEN and IDBG-637256 and ENSBTAG00000009498 and 540786
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