Rabbit Anti-Human PPAR gamma Polyclonal: PerCP

  • Catalog number
    SPC-1308D-PCP
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    A synthesized peptide derived from human PPAR-gamma
  • Antibody s target
    Human PPAR gamma
  • Antibody s full description
    Rabbit Anti-Human PPAR gamma Polyclonal
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    CIMT1 Antibody, GLM1 Antibody, NR1C3 Antibody, Nuclear receptor subfamily 1 group C member 3 Antibody, OTTHUMP00000185032 Antibody, OTTHUMP00000185036 Antibody, Peroxisome proliferator activated nuclear receptor gamma variant 1 Antibody, Peroxisome proliferator activated receptor gamma 1 Antibody, Peroxisome Proliferator Activated Receptor gamma Antibody, Peroxisome proliferator-activated receptor gamma Antibody, PPAR gamma Antibody, PPAR-gamma Antibody, PPARG Antibody, PPARG_HUMAN Antibody, PPARG1 Antibody, PPARG2 Antibody, PPARgamma Antibody
  • Verified applications
    WB, IHC
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects ~56kDa, endogenous levels of total PPAR-gamma
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-1308 was sufficient for detection of PPAR gamma in 10 µg of HuvEc cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Nucleus, Cytoplasm
  • Tissue specificity
    Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary
  • Scientific context
    Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. PPARG is mainly present in adipose tissue, colon and macrophages. Two isoforms of PPARG are detected in the human and in the mouse: PPAR-γ1 (found in nearly all tissues except muscle) and PPAR-γ2 (mostly found in adipose tissue and the intestine)
  • Bibliography
    1. Chandra, V. et al. (2008) Nature 456 (7220): 350–356. 2. Greene ME et al. (1995) Gene Expr. 4 (4–5): 281–99. 3. Elbrecht A. et al. (1996) Biochem. Biophys. Res. Commun. 224 (2): 431–7.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_056953.2
  • Gene number
    5468
  • Protein number
    P37231
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure legend
    Western blot analysis of Human HuvEc cell lysates showing detection of ~57kDa PPAR gamma protein using Rabbit Anti-PPAR gamma Polyclonal Antibody (SPC-1308). Lane 1: Human HuvEc Whole Cell lysates treated with the immunizing peptide. Lane 2: Human HuvEc Whole Cell lysates. Primary Antibody: Rabbit Anti-PPAR gamma Polyclonal Antibody (SPC-1308) at 1:1000. Predicted/Observed Size: ~57kDa. Rabbit Anti-PPAR gamma Antibody used in Western blot (WB) on HuvEc cell lysates (SPC-1308)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugate
    Peridinin chlorophyll (PerCP)
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    PPAR   gamma  
  • Gene symbol
    PPARA
  • Short name
    Rabbit Anti- PPAR gamma Polyclonal: PerCP
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- PPAR gamma Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG
  • Label
    PerCP
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman PPAR g polyclonal: PerCP
  • Alternative technique
    polyclonals, rabbit-anti, antibodies-percp, antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    peroxisome proliferator activated receptor alpha
  • Synonyms gene
  • Synonyms gene name
    • peroxisome proliferative activated receptor, alpha
    • peroxisome proliferator-activated receptor alpha
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1993-11-01
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Peroxisome proliferator activated receptors
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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