-
Immunogen
A synthesized peptide derived from human PPAR-gamma
-
Antibody s target
Human PPAR gamma
-
Antibody s full description
Rabbit Anti-Human PPAR gamma Polyclonal
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
CIMT1 Antibody, GLM1 Antibody, NR1C3 Antibody, Nuclear receptor subfamily 1 group C member 3 Antibody, OTTHUMP00000185032 Antibody, OTTHUMP00000185036 Antibody, Peroxisome proliferator activated nuclear receptor gamma variant 1 Antibody, Peroxisome proliferator activated receptor gamma 1 Antibody, Peroxisome Proliferator Activated Receptor gamma Antibody, Peroxisome proliferator-activated receptor gamma Antibody, PPAR gamma Antibody, PPAR-gamma Antibody, PPARG Antibody, PPARG_HUMAN Antibody, PPARG1 Antibody, PPARG2 Antibody, PPARgamma Antibody
-
Verified applications
WB, IHC
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse, Rat
-
Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects ~56kDa, endogenous levels of total PPAR-gamma
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:1000 dilution of SPC-1308 was sufficient for detection of PPAR gamma in 10 µg of HuvEc cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Nucleus, Cytoplasm
-
Tissue specificity
Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary
-
Scientific context
Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. PPARG is mainly present in adipose tissue, colon and macrophages. Two isoforms of PPARG are detected in the human and in the mouse: PPAR-γ1 (found in nearly all tissues except muscle) and PPAR-γ2 (mostly found in adipose tissue and the intestine)
-
Bibliography
1. Chandra, V. et al. (2008) Nature 456 (7220): 350–356. 2. Greene ME et al. (1995) Gene Expr. 4 (4–5): 281–99. 3. Elbrecht A. et al. (1996) Biochem. Biophys. Res. Commun. 224 (2): 431–7.
-
Released date
6/Sep/2016
-
NCBI number
NP_056953.2
-
Gene number
5468
-
Protein number
P37231
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
-
Representative figure legend
Western blot analysis of Human HuvEc cell lysates showing detection of ~57kDa PPAR gamma protein using Rabbit Anti-PPAR gamma Polyclonal Antibody (SPC-1308). Lane 1: Human HuvEc Whole Cell lysates treated with the immunizing peptide. Lane 2: Human HuvEc Whole Cell lysates. Primary Antibody: Rabbit Anti-PPAR gamma Polyclonal Antibody (SPC-1308) at 1:1000. Predicted/Observed Size: ~57kDa. Rabbit Anti-PPAR gamma Antibody used in Western blot (WB) on HuvEc cell lysates (SPC-1308)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugate
Peridinin chlorophyll (PerCP)
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Gene target
-
Gene symbol
PPARA
-
Short name
Rabbit Anti- PPAR gamma Polyclonal: PerCP
-
Technique
anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- PPAR gamma Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit, Rabbits
-
Isotype
IgG
-
Label
PerCP
-
Species
Human, Humans
-
Alternative name
production species: rabbit Antibody toHuman PPAR g polyclonal: PerCP
-
Alternative technique
polyclonals, rabbit-anti, antibodies-percp, antibodies
-