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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr390 of human PKM2 (AA387-393)
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Antibody s target
Human PKM2 (pTyr390)
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Antibody s full description
Rabbit Anti-Human PKM2 (pTyr390) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
CTHBP Antibody, Cytosolic thyroid hormone binding protein Antibody, Cytosolic thyroid hormone-binding protein Antibody, KPYM_HUMAN Antibody, MGC3932 Antibody, OIP 3 Antibody, OIP-3 Antibody, OIP3 Antibody, OPA interacting protein 3 Antibody, Opa-interacting protein 3 Antibody, p58 Antibody, PK muscle type Antibody, PK muscle type Antibody, PK2 Antibody, PK3 Antibody, PKM Antibody, PKM2 Antibody, pykm Antibody, Pyruvate kinase 2/3 Antibody, Pyruvate kinase 3 Antibody, Pyruvate kinase isozymes M1/M2 Antibody, Pyruvate kinase muscle Antibody, Pyruvate kinase muscle isozyme Antibody, Pyruvate kinase PKM Antibody, Pyruvate kinase, muscle 2 Antibody, TCB Antibody, THBP1 Antibody, Thyroid hormone binding protein 1 Antibody, Thyroid hormone binding protein cytosolic Antibody, Thyroid hormone-binding protein 1 Antibody, Tumor M2 PK Antibody, Tumor M2-PK Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 57.937 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1063 was sufficient for detection of PKM2 (pTyr390) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus
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Tissue specificity
Expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_001193725
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Gene number
5315
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Protein number
P14618
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
PKM
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Short name
Rabbit Anti- PKM2 (pTyr390) Polyclonal: Alkaline Phosphatase
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
Alkaline Phosphatase
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman pyruvate kinase, muscle (pTyr390) polyclonal: Alkaline Phosphatase
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
pyruvate kinase, muscle, CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, poly(A) RNA binding, nuclei, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571
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