Rabbit Anti-Human PI 3 Kinase Class 3 Polyclonal: Streptavidin

  • Catalog number
    SPC-661D-STR
  • Price
    Please ask
  • Size
    100 µg
  • Immunogen
    Synthetic peptide from the N-terminal of Human PI 3 Kinase Class 3
  • Antibody s target
    Human PI 3 Kinase Class 3
  • Antibody s full description
    Rabbit Anti-Human PI 3 Kinase Class 3 Polyclonal
  • Primary research fields
    Cell Signaling, Metabolism, Lipid and lipoprotein Metabolism, Immunology, Innate Immunity, TLR Signalling
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    PI3 kinase type 3 Antibody, PI 3 kinase type 3 Antibody, hVps34 Antibody, Phosphatidylinositol 3-kinase p100 subunit Antibody, Phosphatidylinositol 3 kinase p100 subunit Antibody, PI3K type 3 Antibody, PI3-kinase type 3 Antibody, EC:2.7.1.137 Antibody, PK3C3_HUMAN Antibody, PtdIns-3-kinase type 3 Antibody, PIK3C3 Antibody, VPS34 Antibody, Pik3c3 Antibody, Phosphoinositide 3 kinase class 3 Antibody, Phosphatidylinositol 3-kinase catalytic subunit type 3 Antibody, PtdIns 3 kinase type 3 Antibody, Phosphoinositide-3-kinase class 3 Antibody, Phosphatidylinositol 3 kinase class 3 Antibody, Vps 34 Antibody, Phosphatidylinositol 3 kinase catalytic subunit type 3 Antibody, MGC61518 Antibody, Vps34 Antibody, PI3 kinase class 3 Antibody
  • Verified applications
    WB, ICC/IF
  • Raised in
    Rabbit
  • Antibody s reactivity
    Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~101.5 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-661 was sufficient for detection of PI 3 Kinase Class 3 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome
  • Tissue specificity
    Ubiquitously expressed, with a highest expression in skeletal muscle.
  • Scientific context
    See included datasheet or contact our support service.
  • Bibliography
    Refer to PubMed
  • Released date
    25/Oct/2016
  • NCBI number
    NP_002638.2
  • Gene number
    5289
  • Protein number
    Q8NEB9
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (SPC-661). Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (SPC-661) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite. | Western blot analysis of Rat, Mouse Liver cell lysates showing detection of ~101.5 kDa PI 3 Kinase Class 3 protein using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (SPC-661). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Lane 3: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (SPC-661) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~101.5 kDa. Rabbit Anti-PI 3 Kinase Class 3 antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on C2C12 Cells (Mouse Myoblast cell line) (SPC-661) | Rabbit Anti-PI 3 Kinase Class 3 antibody used in Western blot (WB) on Liver cell lysates (SPC-661)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Short name
    Rabbit Anti- PI 3 Kinase Class 3 Polyclonal: Streptavidin
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    Streptavidin
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman PI 3 phosphorylation catalyst Class 3 polyclonal: biotinilated-binding streptavidin
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee