Rabbit Anti-Human PDGFR alpha (pTyr768) Polyclonal: RPE

  • Catalog number
    SPC-1049D-RPE
  • Price
    Please ask
  • Size
    100 µl
  • Immunogen
    A phospho-specific peptide corresponding to residues surrounding Tyr768 of human PDGFR Alpha (AA765-771)
  • Antibody s target
    Human PDGFR alpha (pTyr768)
  • Antibody s full description
    Rabbit Anti-Human PDGFR alpha (pTyr768) Polyclonal
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    PDGF alpha chain Antibody, PDGF Receptor alpha Antibody, PDGF-R-alpha Antibody, PDGFR 2 Antibody, PDGFR alpha Antibody, PDGFR-alpha Antibody, PDGFR2 Antibody, PDGFRA Antibody, PDGFR-A Antibody, PDGFRA/BCR fusion Antibody, PGFRA_HUMAN Antibody, Platelet derived growth factor receptor 2 Antibody, Platelet derived growth factor receptor alpha Antibody, Platelet derived growth factor receptor alpha polypeptide Antibody, Platelet derived growth factor receptor Antibody, Alpha-type platelet-derived growth factor receptor Antibody, CD140 antigen-like family member A Antibody, CD140a Antibody, CD140a antigen Antibody, MGC74795 Antibody, Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein Antibody, RHEPDGFRA Antibody
  • Verified applications
    WB, AM
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:250); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.025% Thimerosal
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects 122.67 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:250 dilution of SPC-1049 was sufficient for detection of PDGFR alpha (pTyr768) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane, Nucleus
  • Tissue specificity
    Widely expressed. Detected in platelets, brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
  • Scientific context
    PDGFRA is a receptor-tyrosine kinase that is activated upon binding platelet-derived growth factor (PDGF). Ligand binding causes dimerization of the receptor to activate signalling cascades to activate effector substrates such as PIK3R1, PLCG1, and PTPN11. Effector substrates will cause release of calcium from endoplasmic reticulum, by PLCG1, and activation of AKT1, by PIK3R1. This ultimately leads to growth and proliferation in cells by the MAPK and STAT transcription factor activation. PDGFRA is negatively regulated by protein phosphatases to cause inactivation and internalization. Due to PDGFRA's role in cellular growth its dysfunction may lead tumourigenesis. A high number of gastrointestinal stromal tumours have been implicated in PDGFRA dysfunction and constitutive activation. Typically mis-sense or deletion mutations were found to cause constitutively activated PDGFRA. Furthermore, a fusion protein of PDGFRA with BCR can arise from a translocation event with the PDGFRA gene locus. This fusion protein has been implicated in the development of chronic myeloid leukemias.
  • Bibliography
    Refer to PubMed
  • Released date
    29/Aug/2016
  • NCBI number
    NP_006197
  • Gene number
    5156
  • Protein number
    P16234
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    No Data Available
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    The Rabbit Anti- PDGFR alpha (pTyr768) Polyclonal: RPE is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    PDGFR   alpha   pTyr768   RPE  
  • Gene symbol
    RPE, PDGFRB
  • Short name
    Rabbit Anti- PDGFR alpha (pTyr768) Polyclonal: RPE
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    RPE
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman PDGFR a (pTyr768) polyclonal: RPE
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    platelet derived growth factor receptor beta
  • Synonyms gene
  • Synonyms gene name
    • platelet-derived growth factor receptor, beta polypeptide
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Receptor tyrosine kinases
    • CD molecules
    • I-set domain containing
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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