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Immunogen
Synthesized peptide derived from human PCNA.
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Antibody s target
Human PCNA
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Antibody s full description
Rabbit Anti-Human PCNA Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ATLD2 Antibody, cb16 Antibody, Cyclin Antibody, DNA polymerase delta auxiliary protein Antibody, etID36690.10 Antibody, fa28e03 Antibody, fb36g03 Antibody, HGCN8729 Antibody, MGC8367 Antibody, Mutagen-sensitive 209 protein Antibody, OTTHUMP00000030189 Antibody, OTTHUMP00000030190 Antibody, PCNA Antibody, Pcna/cyclin Antibody, PCNA_HUMAN Antibody, PCNAR Antibody, Polymerase delta accessory protein Antibody, Proliferating cell nuclear antigen Antibody, wu:fa28e03 Antibody, wu:fb36g03 Antibodya
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Verified applications
WB, IHC, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:1000), IHC (1:100), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~29kDa, endogenous levels of total PCNA protein.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1307 was sufficient for detection of PCNA in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is a 36 kDa molecule that is highly conserved between species. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
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Bibliography
1. Moldovan GL. et al. (2007) Cell 129 (4): 665–79. 2. Warbrick E. et al. (1998) BioEssays 20 (3): 195–9. 3. Gilljam KM. et al. (2009) The Journal of Cell Biology 186 (5): 645–54.
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Released date
6/Sep/2016
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NCBI number
NP_872590.1
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Gene number
5111
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Protein number
P12004
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HepG2 cell lysates showing detection of ~29kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307). Lane 1: Human HepG2 cells. Lane 2: Human HepG2 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-1307) at 1:1000. Predicted/Observed Size: ~29kDa. Rabbit Anti-PCNA Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1307) | Rabbit Anti-PCNA Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1307)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
PCNA, PCNA-AS1
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Short name
Rabbit Anti- PCNA Polyclonal: FITC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Label
FITC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman PCNA polyclonal: fluorecein
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Alternative technique
polyclonals, rabbit-anti, antibodies, fluorescine
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