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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser144 of human PAK1 (AA141-147)
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Antibody s target
Human PAK1 (pSer144)
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Antibody s full description
Rabbit Anti-Human PAK1 (pSer144) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ADRB2 Antibody, Alpha PAK Antibody, Alpha-PAK Antibody, p21 activated kinase 1 Antibody, p21 protein (Cdc42/Rac) activated kinase 1 Antibody, p21-activated kinase 1 Antibody, p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) Antibody, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) Antibody, p65 PAK Antibody, p65-PAK Antibody, P68-PAK Antibody, PAK alpha Antibody, PAK-1 Antibody, Pak1 Antibody, PAK1_HUMAN Antibody, Paka Antibody, PAKalpha Antibody, Protein kinase MUK2 Antibody, Rac/p21-activated kinase Antibody, Serine/threonine-protein kinase PAK 1 Antibody, STE20 homolog yeast Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 60.661 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1043 was sufficient for detection of PAK1 (pSer144) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Cell Junction, Focal Adhesion, Cell Membrane, Cell Projection, Invadopodium
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Tissue specificity
Highly expressed in the brain and blood cells. Overexpressed in gastric cancer cells and tissues.
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Scientific context
PAK1 is a protein-serine/threonine kinase. Pak1 binds to activated forms of the GTPases Cdc42 and Rac. Upon binding to Cdc42 or Rac, Pak1 is activated and autophosphorylates. Pak1 has dozens of substrates, activating cytoskeletal and transcription pathways that enhance cell motility, proliferation, and survival.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_002567
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Gene number
5058
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Protein number
Q13153
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Conjugation
not conjugated
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Gene target
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Gene symbol
PAK1, PKN1
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Short name
Rabbit Anti- PAK1 (pSer144) Polyclonal: Unconjugated
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
Unconjugated
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman p21 protein (Cdc42/Rac)-activated kinase 1 (pSer144) polyclonal: nonconjugated
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
p21 protein (Cdc42/Rac)-activated kinase 1, PAKalpha, PAK1 and IDBG-65610 and ENSG00000149269 and 5058, transferase activity, nuclei, Pak1 and IDBG-199974 and ENSMUSG00000030774 and 18479, PAK1 and IDBG-642220 and ENSBTAG00000010191 and 533729
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