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Immunogen
Mouse p33 ING1 N-terminal peptide –KLH conjugates
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Antibody s target
Human ING1
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Antibody s full description
Rabbit Anti-Human p33 ING1 Polyclonal
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Primary research fields
Cell Signaling, Epigenetics, Cancer
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Growth inhibitor ING1 Antibody, ING1 Antibody, inhibitor of growth 1 Antibody, p24ING1c Antibody, p33 Antibody, p33ING Antibody, p33ING1b Antibody, p33ING1c Antibody, p47 Antibody, p47ING1a Antibody, tumor suppressor ING1 Antibody
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Verified applications
WB, ICC/IF, ELISA
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse
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Antibody s recommended dilutions for use
WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
TBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
0.25 mg/ml
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Antibody s specificity
Detects ~33kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
0.25 mg/ml was sufficient for detection of SPC-145 in lysates prepared from human melanoma cell lines by western blot analysis.
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Antibody in cell
Nucleus
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
The p33ING1 protein encodes a 33-kD, 294-amino acid protein that displays the characteristics of a tumor suppressor gene. The p33ING1 protein is a regulator of cell cycle, senescence, and apoptosis. It can mediate growth arrest, anchor dependent growth and influence chemo sensitivity (1-4). It also has a proposed role as a growth regulator, which is consistent with its location in the nucleus (5). It has been demonstrated that the ING1 gene has three exons and that four mRNA variants are transcribed from three different promoter regions (p33 ING1, p24 ING1, p27 ING1, p47 IG1) (6, 7). ING1 can possibly influence the above biological functions depending on which variant is expressed (1-4).
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Bibliography
1. Cheung, K.J., Jr. and Li, G. (2002) Int. J. Oncol. 21(6): 1361-1365. 2. Cheung, K.J., Jr. and Li, G. (2002) Exp. Cell Res. 279(2): 291-28. 3. Cheung, K.J., Jr. and Li, G. (2002) Int. J. Oncol. 20(6):1319-1322. 4. Cheung, K.J., Jr. and Li, G. (2001) Exp. Cell Res. 268(1): 1-6. 5. Garkavtsev, I. et al. (1996). Nat Genet. 14(4): 415-420. 6. Gunduz, M. et al. (2000). Cancer Res. 60(12): 3143-6. 7. Jager, D. et al. (1999). Cancer Res. 59: 6197-6204.
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Released date
1/Jan/2011
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NCBI number
NP_036049.2
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Gene number
26356
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Protein number
Q9QXV3
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p33 ING1 Polyclonal Antibody (SPC-145). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p33 ING1 Polyclonal Antibody (SPC-145) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p33 ING1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. | Western blot analysis of Mouse brain cell lysates showing detection of ING1 protein using Rabbit Anti-ING1 Polyclonal Antibody (SPC-145). Primary Antibody: Rabbit Anti-ING1 Polyclonal Antibody (SPC-145) at 1:1000. Left: cell expressed flag-tag. Right: Untagged. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p33 ING1 Polyclonal Antibody (SPC-145). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p33 ING1 Polyclonal Antibody (SPC-145) at 1:50 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p33 ING1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. Rabbit Anti-p33 ING1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-145) | Rabbit Anti-ING1 Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-145) | Rabbit Anti-p33 ING1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-145)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.4
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
