-
Immunogen
Synthesized peptide derived from human p12 INK antibody.
-
Antibody s target
Human p16INK4a
-
Antibody s full description
Rabbit Anti-Human p16INK4a Polyclonal
-
Primary research fields
Cancer, Cell Cycle
-
Antibody s category
Polyclonal Antibodies
-
Antibody s other name
CDKN2A Antibody, CDKN2 Antibody, MTS1 Antibody, Cyclin-dependent kinase inhibitor 2A Antibody, isoforms 1/2/3, Cyclin-dependent kinase 4 inhibitor A Antibody, CDK4I Antibody, p16-INK4a Antibody, Multiple tumor suppressor 1 Antibody
-
Verified applications
WB, ELISA
-
Raised in
Rabbit
-
Antibody s reactivity
Human, Mouse
-
Antibody s recommended dilutions for use
WB (1:1000), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
-
Antibody s purified from
Peptide Affinity Purified
-
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
-
Antibody s concentration
See included datasheet or contact our support service
-
Antibody s specificity
Detects endogenous levels of total p16 INK protein.
-
Storage recommendations
-20°C
-
Shipping recommendations
Blue Ice or 4°C
-
Certificate of analysis
A 1:1000 dilution of SPC-1280 was sufficient for detection of p16INK4a in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
-
Antibody in cell
Cytoplasm, Nucleus
-
Tissue specificity
Widely expressed but not detected in brain or skeletal muscle.
-
Scientific context
p16INK4a is a 16 kDa member of the CDKN2 cyclin-dependent kinase inhibitor family of molecules. It is widely expressed (although not in skeletal muscle) and serves as a negative regulator of cell proliferation. It does so by associating with CDK4 or 6, thereby blocking cyclin binding and subsequent Ser/Thr kinase activity.
-
Bibliography
1. Ohtani N., Yamakoshi K., Takahashi A., Hara E. (2004) J Med Invest. 51(3-4): 146-153.
-
Released date
6/Sep/2016
-
NCBI number
NP_000068.1
-
Gene number
1029
-
Protein number
P42771
-
PubMed number
Refer to PubMed
-
Tested applications
To be tested
-
Tested reactivity
To be tested
-
Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
-
-
Representative figure legend
Western blot analysis of Human HeLa cell lysates showing detection of ~16kDa p16INK4a protein using Rabbit Anti-p16INK4a Polyclonal Antibody (SPC-1280). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-p16INK4a Polyclonal Antibody (SPC-1280) at 1:1000. Predicted/Observed Size: ~16kDa. Rabbit Anti-p16INK4a Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1280)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availabilit
In Stock
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
-
Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Conjugation
HRP Conjugated
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
